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Bent Weeke

Per S Skov

Svend Norn

Histamine is detected or determined selectively in a sample by causing the sample to contact glass microfibers in such a quantitative proportion as will permit the present histamine to bind to the fibers. The fiber bound amount of histamine may be determined by competitive determination in the presence of labelled histamine on the basis of a standard curve, or may be determined directly by conventional coupling reactions. The method is simple and particularly useful for allergy diagnostics because it exhibits good correlation with known fluorometric methods.

Method of detecting or determining histamine in histamine containing

Histamine is detected or determined selectively in a sample by causing the sample to contact glass microfibers in such a quantitative proportion as will permit the histamine present to bind to the fibers. The amount of bound histamine may be determined by competitive determination in the presence of labelled histamine on the basis of a standard curve, or may be determined directly by conventional coupling reactions. The method is simple and particularly useful for allergy diagnostics because it exhibits good correlation with known fluorometric methods.

A specimen of body fluid is applied to an aluminum-hydroxide gel to which a known quantity of antigens is adhered by adsorption. Antibodies in the body fluid may be detected by determining the antigen-antibody complex adsorbed to the aluminum-hydroxide gel by means of marked anti-antibodies. A preparation of antigens adsorbed to an aluminum-hydroxide gel can be standardized by treatment with a known quantity of antibody followed by a determination of the antigen-antibody complex adsorbed to the gel by means of marked anti-antibodies.

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Bronshoj, Denmark

Bent Weeke