National University of Singapore

Bensheng Ju

Tie Yan

Toong Jin Lam

Zhiyuan Gong

Jiangyan He

Yanfei Xu

Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed respectively, were isolated and ligated to the 5' end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultraviolet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patterns, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

Chimeric gene constructs for generation of fluorescent transgenic ornamental fish

Sale of fluorescent transgenic ornamental fish

Sale of transgenic fish that express gene encoding fluorescent protein

Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed, respectively, were isolated and ligated to the 5' end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultra-violet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patters, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.

Growing community of inventors

Singapore, Singapore

Bensheng Ju