Centre National De La Recherche Scientifique

Université Paris Diderot-Paris 7

Université De Bourgogne

Benoît Limoges

Damien Marchal

Murielle Dequaire

Thibaut Defever

Laurent Authier

A method and assembly for electrochemically identifying target nucleotide sequences. The method includes supplying a biological sample that may contain a predetermined target nucleotide sequence; supplying activatable amplification materials comprising free nucleotides to form replicated target nucleotide sequences; supplying an oxido-reducible compound capable of being inserted during replication between the nucleotides forming the replicated target sequences; and activating the activatable amplification materials before applying an electric field to the sample in order to activate the oxido-reducible compound. The replicated target sequences cause inhibition of electrochemical activity of the inserted oxido-reducible compound, and the presence of the predetermined target nucleotide sequence is determined in instances where the electric current decreases.

Method for electrochemically identifying target nucleotide sequences

The invention relates to a method and a collection for the electrochemical detection of target nucleic acid sequences. According to the method, a biological sample that may contain a nucleic acid is provided, said nucleic acid being capable of containing a target sequence, said biological sample being mixed with an oxidizing agent, said target sequence comprising at least one nucleotide base that can be oxidized by said oxidizing agent; complementary means capable of coupling with said target sequence are provided; according to the invention, said complementary means comprise activatable amplification means suitable for replicating said target sequence, said amplification means comprising at least nucleotides which include said nucleotide base, wherein said nucleotides are able to be consumed during replication so as to constitute replicated nucleic acids; and the presence of said target sequence is determined by applying an electric field to said sample and recording the decrease in the electric current.

Method for the electrochemical detection of target nucleic acid sequences

The invention concerns a method for detecting or quantifying a biological substance coupled with a colloidal metal particle by electrochemical detection, characterised in that it comprises a step which consists in dissolving by chemical treatment of said colloidal metal particle, prior to detection.

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Brétigny-sur-Orge, France

Benoît Limoges