Mcgill University

Dna Landmarks Inc.

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Affymetrix, Inc.

Basf Plant Science Gmbh

Her Majesty the Queen in the Right of Canada As Represented by the Minister of Agriculture and Agri-food Soils and Crops Research and Dev

Benoit S Landry

Gregory G Brown

Ronald J Sapolsky

Bertrand Lemieux

Charles Dendy

Wing Cheung

Natasa Formanova

Fang-Ming Lai

Yves Castonguay

Martine Jean

Jin Hua

Martin LaForest

Hua Jin

Louis P Vezina

Antonio F Monroy

Serge Laberge

Louise S O'Donoughue

A method is provided for identifying one or more regions within a genome of an organism of interest that mediate the expression of one or more genes of interest. The method comprises identifying a first organism of interest exhibiting a measurable response to an environmental stimulus; identifying a second organism of interest that lacks or does not exhibit as strong a response to the stimulus as compared with the first organism of interest; crossing the first and second organisms of interest to produce a population of progeny; extracting RNA from each individual population of progeny; quantifying a level of gene expression for one or more genes of interest that are associated with the response to an environmental stimulus; identifying one or more Quantitative Trait Locus (QTL), wherein gene expression level is a quantitative trait, and using one or more markers comprising one or more regulatory sequences to mediate said expression of said one or more genes of interest induced by said environmental stimulus; and identifying the one or more regulatory sequences located at said one or more QTL.

Map-based genome mining method for identifying regulatory loci controlling the level of gene transcripts and products

The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as. Preferably, the nuclear fertility restorer genes are used with the Ogura (ogu) CMS system in

Nuclear fertility restorer genes and methods of use in plants

The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such asPreferably, the nuclear fertility restorer genes are used with the Ogura (ogu) CMS system in

The instant invention provides a method for detecting the presence of a restorer gene in the nuclear genomic DNA of a Brassica plant comprising the use of a probe/primer comprising the sequence of the Brassica glyceraldehyde-3-phosphate dehydrogenase (SEQ ID NO: 1) or a sufficient hybridizing fragment thereof.

Glyceraldehyde-3-phosphate dehydrogenase and nuclear restoration of cytoplasmic male sterility

The invention provides oligonucleotides and their complements that can be used as allele-specific probes or primers for sequencing, oligonucleotide probe hybridization, and allele-specific amplification. Such oligonucleotides can be used, for example, to facilitate genetic distinction between individual plants in plant populations.

Brassica polymorphisms

Growing community of inventors

L'Acadie, Canada

Benoit S Landry