Puridify Ltd.

Cytiva Bioprocess R&d Ab

Benjamin Wallis

Marc Hummersone

Frederic Leroux

William Howard Albert Law

William Law

The invention discloses a functionalised chromatography medium, comprising:

Chromatography media and methods for producing them

Functionalised polymeric chromatography medium, comprising: at least one non-woven sheet comprising one or more polymeric nanofibers having a mean diameter of 10-1000 nm; one or more polymer chains grafted onto the one or more polymeric nanofibers, wherein the polymer chains are poly-glycerol chains comprising glycidol monomer residues or wherein the polymer chains comprise divinylsulfone monomer residues; and at least one ligand group bonded to the one or more polymer chains.

Functionalised chromatography medium comprising polymer nanofibres and process of preparation thereof

The invention discloses a functionalised chromatography medium, comprising: i) at least one non-woven layer () of polymeric nano fibres () comprising a plurality of nanofibre-nano fibre fusion points (); ii) a grafted polymer coating covering the polymeric nanofibres and the nanofibre-nanofibre fusion points; iii) a plurality of ligand groups covalently bound to the grafted polymer coating, wherein the ligand groups are capable of interacting with a target biomolecule.

The present invention provides a functionalised polymeric chromatography medium, prepared by a process which comprises (i) providing a substrate formed of one or more polymer nanofibres, (ii) grafting one or more neutral polymer chains from the substrate, and (iii) contacting the grafted product with a reagent which functionalises the product of step (ii) as a chromatography medium, wherein step (ii) comprises reacting a plurality of compounds of formula and/or its enantiomers, and/or its derivatives of formula (I) and/or enantiomers and/or diastereomers thereof: with one or more functional groups present on the nanofibre substrate, wherein R, R, R, Rand Rmay be the same or different, and are chosen from H, halogen, C-Calkyl, or C-Calkoxy provided that at least one of R, R, R, Ror Ris not hydrogen. 

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Hertfordshire, United Kingdom

Benjamin Wallis