Harvard College

Benjamin W Pruitt

George M Church

Evan R Daugharthy

Richard C Terry

Je-Hyuk Lee

Alejandro Chavez

Suhani D Vora

Brian M Turczyk

Jing Cheng

The present disclosure provides methods and compositions of modulating expression of a target nucleic acid sequence in a cell. The method comprises introducing into the cell a nucleic acid sequence encoding a Cas9 fusion protein and a guide RNA, wherein the Cas9 fusion protein and the guide RNA are expressed and co-localize at a target site and modulate the expression of the target nucleic acid sequence.

Methods of modulating expression of target nucleic acid sequences in a cell

Methods of volumetric imaging of a three-dimensional matrix of nucleic acids within a cell is provided. An automated apparatus for sequencing and volumetric imaging of a three-dimensional matrix of nucleic acids is provided.

Method and apparatus for volumetric imaging

CRISPR/Cas Systems are provided where a tuned guide RNA is used to discriminate between two protospacer sequences of same length that differ by one nucleotide.

Cas discrimination using tuned guide RNA

Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix

The present disclosure provides a number of targeted nucleic acid FISSEQ library construction methods. Targeted FISSEQ can exhibit several benefits, such as enhanced sensitivity and/or shorter assay time in the detection, identification, quantification, and/or determining the nucleotide sequence of the target species, relative to 'random' or 'whole-omic' detection via FISSEQ.

Growing community of inventors

Cambridge, MA, United States of America

Benjamin W Pruitt