Wake Forest University

Benjamin T Corona

George J Christ

Benjamin S Harrison

Justin M Saul

John B Scott

Catherine Ward

M Saul Justin

Provided herein are methods of culturing organized skeletal muscle tissue from precursor muscle cells by cyclically stretching and relaxing said muscle cells on a support in vitro for a time sufficient to produce said organized skeletal muscle tissue, including reseeding said organized skeletal muscle tissue by contacting additional precursor muscle cells to said organized skeletal muscle tissue on said solid support, and then repeating said step of cyclically stretching and relaxing said muscle cells in said support in vitro for time sufficient to enhance the density (i.e., increased number of nuclei and/or number of multinucleated cells) of said organized skeletal muscle tissue on said support.

Methods for making a tissue engineered muscle repair (TEMR) construct in vitro for implantation in vivo

Provided herein are scaffolds and methods useful to promote the formation of functional clusters on a tissue, for example, motor endplates (MEPs) or a component thereof on skeletal muscle cells or tissue, as well as the use of scaffolds so produced for repairing a tissue injury or defect.

Bioscaffolds for formation of motor endplates and other specialized tissue structures

Growing community of inventors

Converse, TX, United States of America

Benjamin T Corona