The University of Chicago

University of Washington

Gsl Biotech LLC

Benjamin S Glick

Daniel E Strongin

Brooke Bevis

David Baker

Michelle Scalley-Kim

Robert Keenan

Rita L Strack

Dibyendu Bhattacharyya

William Stokes

Polynucleotides encoding variant polypeptides of DsRed are provided herein. The DsRed variants have increased bacterial expression, reduced aggregation, increased solubility, shifted emission spectra or increased brightness relative to a wild-type DsRed.

Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation

Nucleic acid compositions encoding rapidly maturing fluorescent proteins, as well as non-aggregating versions thereof (and mutants thereof) as well as the proteins encoding the same, are provided. The proteins of interest are proteins that are fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that, in certain embodiments, they are mutants of wild type proteins that are obtained either from non-bioluminescent, e.g.,, species or are obtained fromnon-(sea pen) species. In certain embodiments, the subject proteins are mutants of wild typesp. 'red' fluorescent protein. Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

Rapidly maturing fluorescent proteins and methods for using the same

The present invention relates to a method for indexing nucleic acid sequences to aid computer based searching of nucleic acid sequences by indexing a nucleotide sequence by the presence of unique Kmers in the sequence. The method of the present invention comprises utilizing an algorithm to automatically index a nucleic acid sequence.

Method for indexing nucleic acid sequences for computer based searching

Disclosed are sequences encoding monomeric variants of DsRed fluorescent proteins and methods of use.

Monomeric red fluorescent proteins

Disclosed are nucleotide sequences encoding monomeric variants of DsRed fluorescent proteins and methods of use thereof.

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Chicago, IL, United States of America

Benjamin S Glick