Purina Animal Nutrition LLC

Benjamin R Trible

Ravin Gnanasambandam

Hasmukh Ambalal Patel

Theodore Karnezos

Samantha Steelman

Nathan Christopher Burke

Emily Kimminau

Nicholas Evans

Diana Ayala

Joseph Dart

Daniel Grum

The present disclosure describes animal feed products formulated to inhibit focal ulcerative dermatitis, and methods of feeding such products to the animals. Feeding methods involve providing poultry with a feed product that includes a base feed and a direct-fed microbial composition. The direct-fed microbial composition includes one or more strains of. The poultry can include cage-free layer hens at risk of developing, or already diagnosed with, focal ulcerative dermatitis. Inhibition of focal ulcerative dermatitis can increase the egg production of the layer hens relative to layer hens fed the same base feed but lacking the direct-fed microbial composition.

Feed compositions and methods for inhibiting focal ulcerative dermatitis

Exosome purification methods involve use of a whey composition as an exosome source. Exosomes are isolated by subjecting the whey composition to a first ultrafiltration, which yields a first permeate and a first retentate. The first retentate may then be subjected to a second ultrafiltration, yielding a second permeate and a second retentate. During the second ultrafiltration, the first retentate may be treated with carbon dioxide. The second retentate may then be subjected to a third ultrafiltration, yielding a third permeate and a third retentate. The third permeate may then be optionally dried to yield an exosome powder.

Methods of purifying exosomes

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New Haven, MO, United States of America

Benjamin R Trible