Hoffmann-La Roche Inc.

F. Hoffmann-La Roche Ag

Benjamin Moritz

Ulrich Goepfert

Peter Becker

Herein is reported a method for determining methylation of a promoter nucleic acid operably linked to a nucleic acid encoding a polypeptide and thereby determining the long-term productivity of a cell. Also an aspect is a method for selecting a cell for producing a polypeptide by determining the methylation of the promoter nucleic acid operably linked to the structural gene encoding the polypeptide.

Method for the selection of a long-term producing cell using histone acylation as markers

The current invention reports a promoter that has the nucleic acid sequence of SEQ ID NO: 02 or SEQ ID NO: 03 which is a human CMV major immediate-early (hCMV-MIE) promoter/enhancer with C to G point mutation at position &#x2212;41 and/or &#x2212;179 relative to the transcription start site. This new promoter is especially useful for the production of polypeptides at large scale as it shows reduced promoter silencing and improved polypeptide production.

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Munich, Germany

Benjamin Moritz