University of California

Align Technology, Inc.

Benjamin Ming Wu

Daniel Takashi Kamei

Yin To Chiu

Garrett L Mosley

Chunhua Li

Robert Eugene Tricca

Eric E Kuo

Amir Abolfathi

Jennifer C Chen

Wenda Carlyle

Philippe De Smedt

Loc X Phan

Kang Ting

Chih-Wei Chang

Wentai Liu

Yi-Kai Lo

B Chia Soo

Zhong Zheng

Fernando Vinuela

Chia Soo

Shunichi Kuroda

Daniel William Bradbury

James C Y Dunn

Ichiro Yuki

Nguyen Khoi Le

Giovanny F Acosta-Vélez

Kuan-Wen Tung

Pei Yu E Chiou

Shin Ting Sherine Frieda Cheung

Arnold Suwarnasarn

Justin P Wagner

David Yuan Pereira

Chloe Michelle Wu

Matthew Foosing Yee

Joshua Anthony Keefe

Christina Caroline Pearce

Amir Adam Dailamy

Vincent K Wong

Yulong Zhang

Wenda C Carlyle

Disclosed herein are devices and methods that use aqueous two phase systems and lateral flow assays to detect target analytes in a sample. These devices and methods may be used to diagnose a disease or condition in a biological sample, such as blood or serum. In addition, these devices and methods may be used to detect allergens in a food samples or contaminants, such as environmental toxins, in water samples. Device and kit components may be conveniently assembled in a portable container and are amenable to actuation in most settings. The devices are simple to use, requiring a non-trained operator to simply add the sample to the device. Conveniently, the time it takes to detect the target analyte is very short. Thus, the devices and methods disclosed herein provide novel and useful means for point-of-care.

Methods and devices for integrating analyte extraction, concentration and detection

The present invention relates to a near-field acoustic platform capable of synthesizing high resolution, arbitrarily shaped energy potential wells. A thin and viscoelastic membrane is utilized to modulate acoustic wavefront on a deep, sub-wavelength scale by suppressing the structural vibration selectively on the platform. This new acoustic wavefront modulation mechanism is powerful for manufacturing complex biologic products.

Arbitrarily shaped, deep sub-wavelength acoustic manipulation for microparticle and cell patterning

The present invention relates in part to a photocurable resin composition comprising a first compound formed from the reaction of a diisocyanate and an isocyanate reactive compound comprising a double bond, a second compound comprising one or more double bonds susceptible to polymerization, and a photoinitiator. The invention also relates in part to a method of making the photocurable resin composition. The invention also relates in part to a method of fabricating an article comprising the steps of providing a photocurable resin composition comprising a first compound formed from the reaction of a diisocyanate and an isocyanate reactive compound comprising a double bond, a second compound comprising one or more double bonds susceptible to polymerization, and a photoinitiator; 3D printing the photocurable resin to form an article; and curing the article. The invention also relates in part to a 3D printed resin article.

Photocurable resin composition, photocurable resin article, and methods of fabricating the article

In various embodiments devices and methods for the detection and/or quantification of clinically relevant pathogens (e.g., bacteria, fungi, viruses, etc.) are provided. In certain embodiments the device comprises a lateral-flow assay that detects the bacterium at a concentration of less than about 6×10cells/mL, less than about 3×10cells/ml, less than about 1×10CFU/mL, or less than about 50 μg/mL. In certain embodiments the device comprises an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a lateral-flow assay (LFA). In certain embodiments the device comprises a flow-through system comprising a concentration component comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a detection component disposed beneath said concentration component.

Methods and devices for analyte collection, extraction, concentration, and detection for clinical applications

In various embodiments methods and devices are provided for the detection and/or quantification of an analyte. In certain embodiments a device is provided comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase where, in use, said first phase solution becomes a leading phase and said second phase solution becomes a lagging phase; a lateral-flow assay (LFA); and a probe and/or a development reagent, where in use, said probe associates with said first phase solution in said leading phase of said ATPS and/or said development reagent associates with said second phase solution in said lagging phase of said ATPS. In certain embodiments a 'one-pot' system of purifying and amplifying a nucleic acid is provided utilizing, e.g., an ATPS and isothermal amplification reagents.

Biomarker concentration and signal amplification for use in paper-based immunoassays and a single platform for extracting, concentrating, and amplifying DNA

It was discovered that hydrogel scaffolds can be used to induce phase separation as aqueous two-phase systems (ATPSs) pass through and/or rehydrate the scaffolds, allowing for concentration of target analyte(s) (e.g., biomolecule(s)) into a particular phase of the ATPS or into a leading front. Accordingly, in various embodiments methods and devices are provided that utilize aqueous two-phase systems and hydrogel scaffolds to improve the sensitivity of assays (e.g., of point-of-care assays) without sacrificing cost or ease of use.

Hydrogel platform for aqueous two-phase concentration of a target to enhance its detection

In various embodiments devices and methods for the detection and/or quantification of clinically relevant pathogens (e.g., bacteria, fungi, viruses, etc.) are provided. In certain embodiments the device comprises a lateral-flow assay that detects the bacterium at a concentration of less than about 6&#xd7;10cells/mL, less than about 3&#xd7;10cells/ml, less than about 1&#xd7;10CFU/mL, or less than about 50 &#x3bc;g/mL. In certain embodiments the device comprises an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a lateral-flow assay (LFA). In certain embodiments the device comprises a flow-through system comprising a concentration component comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution; and a detection component disposed beneath said concentration component.

A gastrointestinal stimulation apparatus and methods with an electronic controller and a flexible and stretchable electrode array with a central branch and orthogonal bilateral branches that wrap around a section of the gastrointestinal tract and can accommodate repetitive contraction and relaxation movements of the tract. Array branches have a flexible spring structure, stimulation electrodes, recording electrodes, sensors controlled by a controller and adhesion nodes that fix the branches to the tissue. The electrode array can sense the normal peristalsis from upstream tissue and produce a stimulus signal to stimulate the incapable intestine section to generate stimulation-induced contractions. Electrodes on the incapable intestine section can be used for stimulation or recording, the recorded signal from the incapable intestine section can be sent back to the electronics to form a closed loop control system. An impedance measurement using current stimulation can be used to capture low frequency contraction signals.

Flexible and stretchable electrodes for gastrointestinal implants

Provided herein is a pharmaceutical composition for treating, preventing or ameliorating a bone or cartilage condition and methods of making and using the same.

Pharmaceutical compositions for treating or preventing bone conditions

Systems and methods are disclosed for intra-oral delivery of drugs.

Systems and methods for intra-oral drug delivery

Growing community of inventors

San Marino, CA, United States of America

Benjamin Ming Wu