The General Hospital Corporation

Benjamin Kleinstiver

J Keith Joung

Vikram Pattanayak

Shengdar Q Tsai

James Angstman

Jason Michael Gehrke

Rebecca Tayler Cottman

Y Esther Tak

Russell T Walton

Alexander Sousa

Keith J Joung

Cas9 (SpCas9) variants with relaxed PAM requirements capable of high-resolution editing for various applications, and methods of use thereof.

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

The present invention relates to methods to improve the absolute rate of homology-directed repair (HDR) and/or to improve the relative rate of HDR compared with non-homologous end joining (NHEJ).

Methods for increasing efficiency of nuclease-induced homology-directed repair

Engineered versions ofCas9 (SpCas9) and SpCas9 variants that have improved on-target editing capabilities, and methods of use thereof.

CRISPR-Cas enzymes with enhanced on-target activity

Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.

Engineered CRISPR-Cas9 nucleases with altered PAM specificity

Drug-inducible, tunable, and multiplexable Clustered Regularly Interspaced Short Palindromic Repeats fromand1 (Cpf1)-based activators, and methods of use thereof.

Inducible, tunable, and multiplex human gene regulation using CRISPR-Cpf1

Engineered CRISPR fromand1 (Cpf1) nucleases with improved targeting range and enhanced on-target activity, and their use in genomic engineering, epigenomic engineering, base editing, genome targeting, genome editing, and in vitro diagnostics.

Variants of Cpf1 (Cas12a) with altered PAM specificity

Engineered CRISPR fromand1 (Cpf1) nucleases with altered and improved target specificity and their use in genomic engineering, epigenomic engineering, genome targeting, genome editing, and in vitro diagnostics.

Variants of CRISPR fromand1 (Cpf1)

Engineered CRISPR-Cas9 nucleases with improved specificity and their use in genomic engineering, epigenomic engineering, genome targeting, and genome editing.

Growing community of inventors

Medford, MA, United States of America

Benjamin Kleinstiver