Metabolic Explorer

Benjamin Gonzalez

Philippe Soucaille

Michel Chateau

Isabelle Meynial-Salles

Olivier Zink

Rainer Figge

Cédric Boisart

Gwénaëlle Bestel-Corre

Philippe Noel Paul Soucaille

Philippe Nöel Paul Soucaille

The present invention concerns a new method of preparation of a strain of evolved micro-organisms for the production of 1,2-propanediol by the metabolism of a simple carbon source, which method comprises the growth under selection pressure in an appropriate growth medium containing a simple carbon source of an initial bacterial strain that has undergone the deletion of the gene tpiA and the deletion of at least one gene involved in the conversion of methylglyoxal (propanal) into lactate, in order to cause, in said initial strain, the evolution of one or more genes involved in the biosynthesis pathway from DHAP to methylglyoxal and then to 1,2-propanediol towards evolved genes that possess an improved '1,2-propanediol synthase activity', the resulting strain or strains of evolved micro-organisms possessing an improved '1,2-propanediol synthase activity' then being selected and isolated. The invention also concerns the initial micro-organisms and the evolved micro-organisms thus obtained, and a method for the preparation of 1,2-propanediol and possibly acetone by culture of said evolved micro-organisms.

Evolved micro-organisms for the production of 1,2-propanediol

The invention relates to optimised micro-organism strains for the biotransformation production of molecules having NADPH-consuming biosynthetic pathways. The inventive strains can be used in NADPH-consuming biotransformation methods. Said strains are characterized in that one or more NADPH-oxidizing activities are limited.

Optimised micro-organism strains for NADPH-consuming biosynthetic pathways

The present invention is directed to a method for the preparation of evolved microorganisms permitting the creation or modification of metabolic pathways. Strains of evolved microorganisms and evolved genes obtained by the method are also provided. The invention is also directed to the use of evolved microorganisms, genes or proteins in a biotransformation process.

Method for the preparation of an evolved microorganism for the creation or the modification of metabolic pathways

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Riom, France

Benjamin Gonzalez