Janssen Biotech, Inc.

Nunc A/s

Harvard College

Lifescan Corporation

Benjamin Fryer

Alireza Rezania

Douglas A Melton

Jeffrey R Millman

Felicia J Pagliuca

Quinn P Peterson

Shelley Nelson

Kostadinka LiLova

Daina Laniauskas

Marcia Blackmoore

Haiyun Wang

Villy Nielsen

Tina Kristensen Marwood

Elizabeth Rosocha

Thomas Brevig

Ya Xiong Chen

Mads Gürtler

Ramie Fung

Michael Saris Segal

J Lee Pellegrino-Gensey

The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

Surface of a vessel with polystyrene, nitrogen, oxygen and a static sessile contact angle for attachment and cultivation of cells

The present invention provides a method for increasing the expression of MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage comprising the steps of culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin-dependent kinase inhibitor to cause an increase in expression of MAFA.

Methods for making pancreatic endocrine cells

The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation.

Methods for suspension cultures of human pluripotent stem cells

The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation. Specifically, the invention discloses methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.

Method for making human pluripotent suspension cultures and cells derived therefrom

Methods, surface modified plates and compositions for cell attachment, cultivation and detachment

The present invention is directed to methods for the growth, expansion and differentiation of pluripotent stem cells on planar substrates lacking an adlayer and a feeder cell layer.

Methods and compositions for cell attachment and cultivation on planar substrates

The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells, wherein greater than 80% of the cells in the population express markers characteristic of the definitive endoderm lineage.

Differentiation of human embryonic stem cells

Differentiation of human pluripotent stem cells

The present invention provides a method for increasing the expression of MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage comprising the steps of culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin-dependant kinase inhibitor to cause an increase in expression of MAFA.

Growing community of inventors

Skillman, NJ, United States of America

Benjamin Fryer