Hadasit Medical Research Services and Development Ltd.

Es Cell International Pte Ltd

Cell Cure Neurosciences Ltd

Benjamin Eithan Reubinoff

Martin Frederick Pera

Nir Netzer

Tamir Ben-Hur

Eyal Banin

Orna Singer

Michal Aharonowiz

Sharona Cohen Evenram

Limor Matzrafi

Nurit Yachimovich-Cohen

Etti Ben Shushan

Alex Obolensky

Osnat Bohana-Kashtan

Ofer Wiser

Ariffeen Bongso

Martin Federick Pera

Hanita Khaner

Masha Gorshtein

Alan Osborne Trounson

Shelly Tannenbaum

Chui-Yee Fong

Yitzchak Hemo

Debora Steiner

Ruslana Alper-Pinus

Masha Idelson

Pavel Itsykson

Ithai Waldhorn

Ofira Einstein

Charles Sherard Irving

Talya Mordechai Daniel

A method of generating photoreceptor cells is disclosed. Cell populations comprising photoreceptor cells and uses thereof are also disclosed.

Photoreceptor cells for the treatment of retinal diseases

A method of generating retinal pigment epithelium cells is disclosed. Cell populations comprising same and uses thereof are also disclosed.

Preparation of retinal pigment epithelium cells

A method of generating retinal pigment epithelial (RPE) cells is disclosed. The method comprises: (a) culturing human pluripotent stem cells in a human feeder cell-conditioned medium to obtain a cultured population of human pluripotent stem cells; (b) culturing said cultured population of human pluripotent stem cells in a medium comprising a differentiating agent to obtain differentiating cells; and (c) culturing said differentiating cells in a medium comprising one or more members of the TGF&#x3b2; superfamily.

Large scale production of retinal pigment epithelial cells

An article of manufacture is disclosed which comprises at least two populations of autosomal-identical induced pluripotent stem cells (iPSCs), wherein the complement of sex chromosomes of the first population of the at least two populations is non-identical to the complement of sex chromosomes of the second population of the at least two populations. Uses thereof and methods of generating same are also disclosed.

Autosomal-identical pluripotent stem cell populations having non-identical sex chromosomal composition and uses thereof

A method of selecting retinal pigmented epithelial (RPE) cells from a mixed population of cells is disclosed. The method comprises:

Methods of selecting retinal pigmented epithelial cells

The present invention relates to the generation of neural cells from undifferentiated human embryonic stem cells. In particular it relates to directing the differentiation of human ES cells into neural progenitors and neural cells and the production of functioning neural cells and/or neural cells of a specific type. The invention also includes the use of these cells for the treatment of neurological conditions such as Parkinson's disease.

Generation of neural stem cells from undifferentiated human embryonic stem cells

Provided is a method including providing a population of cells including a target type of differentiated cells having a pre-identified cytoskeletal profile and at least one cell selected from undifferentiated cells, differentiating cells and differentiated cells being different from the target type of differentiated cells; and incubating the population of cells with a cytotoxic agent, in an amount and for a time period effective to form a modified population of cells including predominantly or consisting essentially of the target type of differentiated cells. The pre-identified cytoskeletal profile can include the presence of class III &#x3b2;-tubulin on neuronal cells and the population of cells includes neural cells and neuronal cells.

Methods of obtaining cell populations enriched with desired cells

The present invention concerns the use of a population of cells comprising: (a) neural precursor cells committed to an oligodendroglial fate; (b) uncommitted neural precursor cells (c) differentiated oligodendrocytes; or (d) a combination of any one of (a) to (c) for the treatment of CNS autoimmune diseases, or for the preparation of a pharmaceutical composition for treating CNS autoimmune diseases, the population of cells being derived from human pluripotent stem cells. The invention also provides methods for obtaining such populations of cells, namely, neural precursor cells committed to an oligodendroglial fate as well as differentiated oligodendrocytes which then can be used in the treatment of CNS autoimmune diseases. A preferred autoimmune disease in the context of the present invention is multiple sclerosis where the population of cells is administered to the CNS for local treatment of the disease.

Human stem cell-derived neural precursors for treatment of autoimmune diseases of the central nervous system

Provided are systems and methods for providing human cell cultures. Further provided are cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods for maintenance and propagating of stem cells in an undifferentiated state as well as for the development of somatic cells cultures from stem cells, the somatic cell cultures being free of extraembryonic cells.

Stem cells culture systems

The present invention concerns RPE cells obtainable by directed differentiation from stem cell, particularly, human stem cells. It has been specifically found that culturing stem cells in the presence of one or more member of the TGF&#x3b2; superfamily, such as Activin A) induced directed differentiation into mature and functional RPE cells. This was evidenced by the expression of markers specific to mature RPE cells, including MiTF-A, RPE65 or Bestrophin). In accordance with one particular embodiment, the cells are a priori cultured with nicotinamide (NA) which was found to augment the cells' response to the inductive effect of the one or more member of the TGF&#x3b2; superfamily. The invention also provides methods of performing the directed differentiation, as well as methods for use of the resulting RPE cells.

Retinal pigment epithelial cells differentiated from embryonic stem cells with nicotinamide and activin A

The present disclosure provides methods for maintaining and propagating undifferentiated pluripotent stem cells (SC) in suspension. The methods comprise culturing such SC in a non-adherent culture dish under conditions comprising a basic serum free medium and one or more of a basic medium, a serum replacement, an extra cellular matrix component and a factor supporting expansion of said SC. A specific and preferred culture condition comprise supplementing Neurobasal&#x2122; medium with KO serum replacement (KOSR). These conditions allowed for large scale and long term propagation of undifferentiated pluripotent SC. The culture system comprising suspended undifferentiated pluripotent SC were found to have many applications including in methods for directed as well as spontaneous differentiation of the SC into somatic cells. Also disclosed herein is a method of deriving SC, preferably human embryonic SC from human embryos via the formation of cell clusters.

Undifferentiated stem cell culture systems

Growing community of inventors

Moshav Bar-Giora, Israel

Benjamin Eithan Reubinoff