Massachusetts Institute of Technology

The Brigham and Women's Hospital, Inc.

Benjamin E Mead

Robert Samuel Langer

Jeffrey Michael Karp

Alexander K Shalek

Jose Ordovas-Montanes

Disclosed here is a generally applicable framework that utilizes massively-parallel single-cell RNA-seq to compare cell types/states found in vivo to those of in vitro models. Furthermore, Applicants leverage identified discrepancies to improve model fidelity. Applicants uncover fundamental gene expression differences in lineage-defining genes between in vivo systems and in vitro systems. Using this information, molecular interventions are identified for rationally improving the physiological fidelity of the in vitro system. Applicants demonstrated functional (antimicrobial activity, niche support) improvements in Paneth cell physiology using the methods.

Growing community of inventors

Cambridge, MA, United States of America

Benjamin E Mead