Ifp Energies Nouvelles

Stichting Dienst Landbouwkundig Onderzoek

Benjamin Clement

Remy Marchal

Caroline Aymard

Rejane Dastillung

Marcel Ropars

Ana Maria Lopez Contreras

Sandra Menir

Florent Collas

Pieternel Am Claassen

Rémy Marchal

Pieternel A M Claassen

This invention describes a process for the production of second generation alcohols and/or solvents starting from cellulosic or lignocellulosic biomass wherein the lignocellulosic or cellulosic biomass undergoes a pretreatment before being converted into ethanol after an enzymatic hydrolysis and an ethylic fermentation, wherein glucidic polymers of the pretreated plant are hydrolyzed by cellulases; microorganisms that primarily use hexoses and preferably glucose and mannose are used for the ethylic fermentation; wines, with or without separation of materials in suspension are extracted by distillation; pentoses in the vinasses are fermented by a solventogenic microorganism into a butyl wine, and at least one portion of the butyl wine is recycled upstream from enzymatic hydrolysis.

Method for producing ethanol and solvents from lignocellulosic biomass including the recirculation of a butyl wine obtained by fermenting pentoses

The expression vector includes: the nucleic acids coding for the polypeptides forming a polypeptide complex having an enzyme activity allowing acetoacetyl-CoA to be converted to acetoacetate; optionally, at least one nucleic acid coding for a polypeptide having an enzyme activity allowing acetoacetate to be converted to acetone; and at least one nucleic acid coding for a polypeptide having an enzyme activity allowing acetone to be converted to isopropanol; the expression of the nucleic acids being controlled by a single constitutive promoter located upstream of the abovementioned nucleic acids.

Production of isopropanol by improved recombinant strains

Growing community of inventors

Saint Maur des Fosses, France

Benjamin Clement