Ventana Medical Systems, Inc.

Oregon Health & Science University

Pfizer Corporation

Benjamin C Stevens

Michael Otter

David Chafin

Karl Eric Garsha

Daniel Bauer

Nathan Crum

Taylor J Shingler

Michael S Barnes

Joe W Gray

Jerome W Kosmeder, Ii

Christophe Chefd'hotel

Thomas M Grogan

Eric J May

Esteban Roberts

Kandavel Shanmugam

Brian Jay Druker

Jefferson Taft

Thomas Jacob

Franklin Ventura

Joseph M Jakubowski

Damien Ramunno-Johnson

Tothu Tania Vu

Angel Guzman-Perez

Jeffrey Allen Pfefferkorn

Meihua Mike Tu

Kevin James Filipski

Mary Theresa Didiuk

Stacey Stanislaw

Timothy James Keller

Tania Vu

Esther C Lee

Elizabeth Pigman

Vinnie Petre

Alastair Laing

Karl Garsha

Frank Ventura

Brian Jay Drucker

Joe Gray

Ii Jerome W Kosmeder

Marvin Arthur Petre

Tothu Vu

Taylor Shingler

Timothy Keller

Tothu (tania) Vu

Frank R Ventura

Karl E Garsha

Christophe Chefd´hotel

Stanislaw Stacey

A tissue sample that has been removed from a subject can be evaluated. A change in speed of the energy traveling through the sample is evaluated to monitor changes in the biological sample during processing. The monitoring can detect movement of fluid with the sample and cross-linking. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy.

Systems and methods for monitoring tissue sample processing

Automated systems to make target compounds from slide stainer waste streams inactive utilizing advanced oxidation processes are described herein. Advanced oxidation processes are promoted by UV irradiation and further accelerated by use of radical initiators, such as hydrogen peroxide. The automated systems further include mechanisms for segregating components of the waste streams.

Passive, gravity-driven system for treatment of an effluent in a diagnostic system

The subject disclosure presents systems and computer-implemented methods for determining an acoustic time-of-flight (TOF) of sound waves through a sample material with greater accuracy and in a more repeatable fashion, by invoking one or more of an envelope generation for an error function, fitting a non-linear curve to an ultrasound frequency sweep, or performing a clustered piece-wise linear regression on individual linear parts of the ultrasonic frequency sweep. The systems and methods are useful for, among other things, monitoring diffusion of fluids through porous materials, such as tissue samples.

Accurately calculating acoustic time-of-flight

Methods, systems, and apparatuses for detecting and describing heterogeneity in a cell sample are disclosed herein. A plurality of fields of view (FOV) are generated for one or more areas of interest (AOI) within an image of the cell sample are generated. Hyperspectral or multispectral data from each FOV is organized into an image stack containing one or more z-layers, with each z-layer containing intensity data for a single marker at each pixel in the FOV. A cluster analysis is applied to the image stacks, wherein the clustering algorithm groups pixels having a similar ratio of detectable marker intensity across layers of the z-axis, thereby generating a plurality of clusters having similar expression patterns.

Methods, systems, and apparatuses for quantitative analysis of heterogeneous biomarker distribution

A tissue sample that has been removed from a subject can be properly fixed for evaluation using the disclosed transporter assembly for carrying a tissue sample and method for fixing an unfixed tissue sample. In one embodiment, the disclosed assembly includes a transport container, a fixative in the transport container, and a cooling device that reduces and/or maintains the temperature of the fixative to perform a pre-soaking process at a temperature of less than about 7&#xb0; C. The pre-soaking process can, for example, be performed during sample transport or during extended periods of storage, such as over a weekend.

Transporter systems, assemblies and associated methods for transporting tissue samples

The subject disclosure presents systems and computer-implemented methods for calculating the diffusivity constant of a sample using acoustic time-of-flight (TOF) based information correlated with a diffusion model to reconstruct a sample's diffusivity coefficient. Operations disclosed herein such as acoustically determining the phase differential accumulated through passive fluid exchange (i.e. diffusion) based on the geometry of the tissue sample, modeling the impact of the diffusion on the TOF, and using a post-processing algorithm to correlate the results to determine the diffusivity constant, are enabled by monitoring the changes in the speed of sound caused by penetration of fixative such as formalin into several tissue samples. A tissue preparation system may be adapted to monitor said diffusion of a tissue sample and determine an optimal processing workflow.

Obtaining true diffusivity constant

Immunohistochemistry (IHC) and in situ hybridization (ISH) have the aim of detecting, localizing and quantifying certain analytes for various diagnostic purposes. The quality of the stains which are analyzed may deviate for various reasons. Therefore, the present invention provides a method and system for assessing the stain quality and for establishing objective criteria for assessing the stain quality for application in the fields of in-situ hybridization and immunohistochemistry. In one possible embodiment, the invention comprises the steps of unmixing multi-spectral image data of a tissue specimen to obtain analyte intensity images, each analyte intensity image comprising signals from a single stain, computing metrics based on the analyte intensity images, wherein the metrics are uniformity, distribution and/or dispersion of pixel intensity values in the analyte intensity images and assessing a stain quality of a slide by comparing the computed metrics to pre-determined cutoff values regarding uniformity, distribution and/or dispersion of pixel intensity, wherein the stain quality of the slide is assessed as acceptable if the computed metrics meet or exceed the pre-determined cutoff values, and wherein the stain quality of the slide is assessed as unacceptable if the computed metrics do not meet the pre-determined cutoff values. In order to establish objective criteria for assessing stain quality, in one possible embodiment, the method and system includes the step of deriving cut-off values for uniformity, distribution and/or dispersion of pixel intensity by combining the computed metrics based on the analyte intensity images with pre-established data quantifying the stain quality.

Descriptive measurements and quantification of staining artifacts for in situ hybridization

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Oro Valley, AZ, United States of America

Benjamin C Stevens