Tet Systems Gmbh & Co., Kg

Academisch Medisch Centrum Bij De Universiteit Van Amsterdam

Benjamin Berkhout

Atze Taede Das

Hergen Spits

Jurgen E Seppen

David M Markusic

Kees Weijer

Provided is an rtTA and single chain rtTA variants and uses thereof for inducible expression of a nucleic acid of interest. Nucleic acid molecules comprising an improved rtTA and/or sc rtTA sequence according to the invention are also provided, as well as vectors, replicons and cells comprising such nucleic acid molecules.

Inducible expression systems

The invention provides a method for producing a stabilized cell of interest, said method comprising providing a stem cell and/or a precursor cell of said cell of interest with a nucleic acid sequence which, when present in said cell of interest, is capable of stabilizing said cell of interest, providing a non-human animal with said stem cell and/or precursor cell, allowing generation of said cell of interest in said animal, and obtaining said cell of interest. Said animal is preferably provided with a human stem cell and/or human precursor cell, allowing production of a human cell line.

Means and methods for producing a stabilized cell of interest

Growing community of inventors

Naarden, Netherlands

Benjamin Berkhout