Pioneer Hi-bred International, Inc.

Other

Pioneer Hi-bred Int'l Inc.

Columbia University

Benjamin A Bowen

Laura Ann Tagliani

David J Peterson

Christopher L Baszczynski

Xun Wang

Timothy George Helentjaris

Kanwarpal Singh Dhugga

Gary A Sandahl

Bruce J Drummond

William James Gordon-Kamm

John A McElver

Jonathan P Duvick

Zuo-Yu Zhao

Guihua Lu

Pramod B Mahajan

Tong Zhu

Jacob T Gilliam

Wesley B Bruce

Lynne Eileen Sims

Joyce R Maddox

Larry R Beach

Tracy A Rood

Hongyu Wang

Margit C Ross

Rodney J Rothstein

Susan J Martino-Catt

Grace Marie St Clair

Mark D Chamberlin

John H Duesing

Carl R Simmons

Keith S Lowe

Dwight T Tomes

Christopher Jay Scelonge

Dennis L Bidney

Michael D Miller

Robert B Meeley

Jinrui Shi

Dorothy A Pierce

Timothy W Fox

Lijuan Wang

Kathryn K Lappegard

David D Songstad

William Gordan-Kamm

Christopher L Baszcynski

John A Arbuckle

Mike Miller

Gary Sandahl

Compositions for the targeted integration of nucleotide sequences into a plant are provided. Plants and plant cells having multiple transfer cassettes stacked at precise locations in the genome of a plant or plant cell are provided. In specific embodiments, the plant or plant cell comprises at least two transfer cassettes. The first transfer cassette comprises a first nucleotide sequence of interest flanked by a first and a second recombination site, wherein the first and the second recombination sites are non-identical. The second transfer cassette comprises a second nucleotide sequence of interest flanked by the second recombination site and a third recombination site, wherein the third recombination site is non-identical to the first and said second recombination site. The second recombination site is shared between the first and the second transfer cassette, such that the genome of the plant cell comprises in the following order the first recombination site, the first nucleotide sequence of interest, the second recombination site, the second nucleotide sequence of interest, and the third recombination site.

Compositions for the targeted insertion of a nucleotide sequence of interest into the genome of a plant

Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.

Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant

Compositions and methods for genetic modification of plants

Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.

Compositions and methods to stack multiple nucleotide sequences of interest in the genome of a plant

The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.

Maize cellulose synthase encoding sequences and uses thereof

Cellulose synthase encoding nucleic acids and uses thereof

Methods to find optimal integration sites within a plant genome are provided. More particularly, a plant is transformed with a target site having an expression cassette comprising a nucleotide sequence operably linked to a promoter active in the plant. The target site is flanked by non-identical recombination sites. Transformed protoplast, tissues, or whole plants can be tested to determine the levels of activity of the inserted gene. By comparison of cellular activities of the gene in different insertion sites, preferred integration sites may be found wherein the gene is expressed at high or acceptable levels. These plants can then be utilized with subsequent retargeting techniques to replace the nucleotide sequence with other genes or nucleotide sequences of interest contained in a transfer cassette.

Compositions and methods for locating preferred integration sites within the genome of a plant

Compositions comprising non-identical recombination sites are provided. Compositions include plants, plant parts and plant cells comprising transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites.

Compositions comprising non-identical recombination sites

Compositions and methods to reduce the complexity of transgene integration into the genome of a plant

Maize cellulose synthases encoding nucleic acids and uses thereof

The present invention provides methods and compositions relating to altering lignin biosynthesis content and/or composition of plants. The invention provides isolated nucleic acids and their encoded proteins which are involved in lignin biosynthesis. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

Genes encoding enzymes for lignin biosynthesis and uses thereof

Methods and compositions for altering a nucleotide sequence of interest in a plant is provided. The method involves introducing a chimeric oligonucleotide into a plant cell, wherein the oligonucleotide comprises at least two intervening blocks of RNA residues with intervening DNA residues. The RNA blocks are homologous to a plant nucleotide sequence. The chimeric oligonucleotide is capable of folding to form a duplex oligonucleotide. The chimeric oligonucleotide is maintained within the nucleus of the plant whereby the alteration is introduced into the target sequences of the plant genome.

Targeted manipulation of genes in plants

Growing community of inventors

Des Moines, IA, United States of America

Benjamin A Bowen