Nanyang Technological University

Bengang Xing

Tingting Jiang

Rongrong Liu

Xianfeng Huang

Teck Peng Loh

Yanmei Yang

Chungyen Ang

Ata Abbas

Junxin Aw

Qing Shao

There is provided a compound of formula I, having the structure: wherein Rto Rhave the meanings given in the description. 

Allenamide as an orthogonal handle for selective modification of cysteine in peptides and proteins

The present invention relates to compounds for and a method of detecting beta-lactamase activity in a sample. The sample is contacted with a nanoparticulate tag. The nanoparticulate tag comprises a metal or a combination of metals, or it comprises a nanotube of a metal, boron nitride and/or carbon. The respective metal is capable of forming one of a covalent bond, a coordinative bond and a non-covalent interaction with a thio or a seleno group. The sample is contacted with a compound of one of general formulas (I)-(III) and (VII)-(IX). At least one beta-lactam moiety of the compound is cleaved by the beta-lactamase activity in the sample. As a result a cleavage moiety Z-A-Z, Z-A-Z&#x2014;R, Z-A-Z&#x2014;R, Z-A-Z&#x2014;R, Z-A-Z&#x2014;Ror Z-G-N(R)Ris released that is immobilized on the surface of the nanoparticulate tag by a covalent bond via a Z atom. The presence of beta-lactamase activity is determined based on the presence of the cleavage moiety immobilized onto the surface of the nanoparticulate tag.

Methods and compounds for detecting beta-lactamase activity

The present invention relates to compounds for and a method of detecting beta-lactamase activity in a sample. The sample is contacted with a nanoparticulate tag. The nanoparticulate tag comprises a metal or a combination of metals, or it comprises a nanotube of a metal, boron nitride and/or carbon. The respective metal is capable of forming one of a covalent bond, a coordinative bond and a non-covalent interaction with a thio or a seleno group. The sample is contacted with a compound of one of general formulas (I)-(III) and (VII)-(IX). At least one beta-lactam moiety of the compound is cleaved by the beta-lactamase activity in the sample. As a result a cleavage moiety Z-A-Z, Z-A-Z&#x2014;R, Z-A-Z&#x2014;R, Z-A-Z&#x2014;R, Z-A-Z&#x2014;Ror Z-G-N(R)Ris released that is immobilised on the surface of the nanoparticulate tag by a covalent bond via a Z atom. The presence of beta-lactamase activity is determined based on the presence of the cleavage moiety immobilized onto the surface of the nanoparticulate tag.

The present invention relates to a compound of the general formula (I) useful in the determining the presence, amount or activity of an enzyme in living cells, a method of preparing said compounds and a kit thereof.

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Bengang Xing