Becton Dickinson and Company Limited

Accelerate Diagnostics, Inc.

Ben Turng

Dwight Livingston

William L Richards

Wayne A Showalter

Liping Feng

Timothy Roy Hansen

Adrien P Malick

Michael A Brasch

Timothy M Wiles

Ammon David Lentz

Phillip Charles Halbert

Austin Micheil Ashby

Steven W Metzger

Kurt Reinhardt

Patrick Shawn Beaty

Robert Edward Armstrong

Strett Roger Nicolson

Susan M Kircher

William B Brasso

Julie L Rosales

Glen Richard Davis

Vanda White

Dyan Luper

Brent Ronald Pohl

John D Mantlo

Charles C Yu

Ming-hsiung Yeh

Mei Yang-Woytowitz

John Thulin Page

Donald Ray Callihan

Curtis M Gosnell

Eric Ursprung

Xiaofei Chang

Song Shi

Ming-Hsiung Yeh

Kevin David Marshall

Andrew Ghusson

Matthew Ketterer

Paul Richards

Kevin Bailey

Matthew Mette

Josh Harrison

Jasmin Cote

Oleg Gusyatin

Alena Shamsheyeva

Gary F Hershner

Kenneth Robert Hance

Landon Prisbrey

Meghan Mensack

James Y Zhou

John Thuin Page

Dulini Gamage

Elke Allers

Carlos Michel

Patrick R Murray

John P Douglass

Jeffery H Bruton

Gretta Campbell

Solène Bourgeois

Gerard Lotz

Donald R Callihan

Shawn Beaty

An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel, a vessel insert, and a cap element. The vesselhas a body portionfor receiving a biological sample. The vessel insertreceives at least one reagent therein. Preferably, the vessel insertis received in a portionof the vessel. The cap elementis attached to the vesselto secure the vessel insertin the vessel. During use, the vessel insertis adapted to release its contents when the biological sample is introduced into the body portionof the vesselupon application of an intermixing force to the vessel insert. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

Blood culture bottles with mechanisms for controlled release of substances into culture media

An automated system for identifying in a biological sample microorganisms and their antimicrobial susceptibility (AST). The system provided an automated platform for preparing, from a single biological sample, inoculates for both ID and AST. The system loads a plate for ID testing as samples are being prepared for AST testing. The system tracks the sample and the inoculates from the samples to link the test results to the sample and the patients from whom the sample was obtained.

Method and apparatus for identification of bacteria

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

Formulations and process for isolating viable microorganisms from positive blood cultures

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

Methods of the invention include the isolation of intact, viable microorganism(s) from positive blood culture ('PBC') samples for use in downstream analyses such as identification and antimicrobial susceptibility testing ('AST'). The methods involve collecting a portion of the PBC sample, adding a choline-containing solution, lysing the blood cells, isolating the viable microorganism, and performing downstream analysis of the isolated, viable microorganism. The methods can be applied to a variety of gram-positive bacteria, gram-negative bacteria, and/or yeast, and particularly to strains of

Method for direct and rapid identification of microorganisms and antimicrobial susceptibility testing from positive blood cultures

Growing community of inventors

Tucson, AZ, United States of America

Ben Turng