Institut Pasteur

Medical Research Council

Assistance Publique

Université Pierre Et Marie Curie (Paris Vi)

Universite De Berne

Universite Paris-vi

Beate Heym

Stewart T Cole

Ying Zhang

Douglas B Young

Nadine Honore

Amalio Telenti

Thomas Bodmer

A nucleotide sequence encoding a katG/lacZ fusion protein is useful for assaying the enzymatic activity of the katG gene product. A process of selecting a compound that is toxic against an isoniazid-resistant mycobaterial strain comprises incubating a catalase peroxidase enzyme with an isoniazid to produce a compound that restores isoniazid susceptability to the isoniazid-resistant mycobaterial strain.

Rapid detection of antibiotic resistance in mycobacterium tuberculosis

Multi-drug resistant strains of Mycobacterium tuberculosis represent a considerable threat to public health worldwide. Resistance to isoniazid (INH), a key component of anti-tuberculosis regimens, is often associated with loss of catalase activity and virulence. The katG gene, encoding HPI catalase-peroxidase, mediates INH-sensitivity and that the high level resistance encountered clinically may be due to deletions, insertions or point mutations which reduce or eliminate the expression of the catalase gene in the chromosomal region encompassing katG. INH-resistant strains of Mycobacterium tuberculosis are detected by nucleic acid hybridization with a unique nucleic acid sequence or by amplification techniques.

Nucleic acid probes, sequences and methods for detecting mycobacterium

Rapid detection of isoniazid resistance in mycobacterium tuberculosis

Growing community of inventors

Ville d'Avray, France

Beate Heym