The United States of America as Represented by the Secretary of

Beate G Rose

Larry H Stanker

John R DeLoach

Carol Kamps Holtzapple

Hybridoma cell lines have been generated which produce and secrete monoclonal antibodies which selectively bind to the ceftiofur. These hybridomas may be obtained by using as an immunization agent or immunogen, desfuroyl ceftiofur which has been conjugated to an immunogenic carrier. Ceftiofur in biological samples may be detected and quantified by contacting the sample with the antibodies to form a ceftiofur/antibody immunocomplex when ceftiofur is present, which immunocomplex may then be detected. The monoclonal antibodies may also be incorporated into kits for the detection and quantification of ceftiofur.

Growing community of inventors

Chapel Hill, NC, United States of America

Beate G Rose