Iowa State University Research Foundation Inc

Omegachea

Basil J Nikolau

Marna Yandeau-Nelson

Patrick S Schnable

Fuyuan Jing

Eve Syrkin Wurtele

David J Oliver

Huanan Jin

Tsui-Jung Wen

Shivani Garg

Isabelle Lutziger

Robert Behal

Jinshan Ke

Jerry L Johnson

Carolyn C Allred

Beth Fatland

Donald S Robertson

Ludmila Rizhsky

Joel D Hansen

Yiji Xia

Xiaojie Xu

Xiaochen Yu

A method of increasing production of fatty acids comprising introducing into a host and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) fromor a mutant thereof; a method of making a mutantacyl-ACP TE; a method of making a chimericacyl-ACP TE; a nucleic acid molecule comprising a nucleotide sequence encoding a mutant acyl-ACP TE or a chimericacyl-ACP TE; a host comprising the nucleic acid molecule; a mutant acyl-ACP TE or chimericacyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE for at least one of its substrates comprising introducing into the plant acyl-ACP TE a substrate specificity-altering mutation; and a method of altering the level of activity of a plant acyl-ACP TE.

Materials and methods for using an acyl-acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis

A mutant, which does not express a functional KASIIIA and/or KASIIIB, and method of making; a mutant, which does not express a functional PhaC1, PhaC2, and/or PhaC3, and method of making; method of characterizing substrate specificity of KASIII; method of making mutant KASIII with altered substrate specificity and/or altered level of activity and nucleic acid, vector, host cell/organism, and mutant KASIII; an in vitro, high-throughput spectrophotometric method of assaying KASIII activity; and materials and methods for using KASIII for production of bi-functional fatty acids and the materials so produced.

Materials and methods for characterizing and using a 3-ketoacyl-acyl carrier protein (ACP) synthase III (KASIII) for production of bi-functional fatty acids

A method of producing bi-functional fatty acids comprising introducing into a host cell or organism, which comprises one or more &#x3c9;- or &#x3c9;-1 functionalized acyl-CoAs, and expressing therein a KASIII, which can use one or more of the &#x3c9;- or &#x3c9;-1 functionalized acyl-CoAs as a substrate; a method of producing a &#x3c9;-1 hydroxy branched fatty acid, a &#x3c9;-1 branched fatty acid, or a combination thereof by culturing a mutant, which does not express a functional KASIII from the endogenous fabH gene and expresses a phaA and a phaB and a functional exogenous KASIII; and a mutant, a method of making the mutant, a culture comprising the mutant, and a composition comprising &#x3c9;-1 hydroxy branched fatty acids, a &#x3c9;-1 branched fatty acids, or a combination thereof obtained from the culture.

Materials and methods for production of bi-functional fatty acids in recombinant bacteria

Materials and methods for characterizing and using KASIII for production of bi-functional fatty acids

A method of increasing production of fatty acids comprising introducing into a host cell or organism and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) fromor a mutant thereof; a method of making a mutantacyl-ACP TE; a method of making a chimericacyl-ACP TE; a nucleic acid encoding a mutant acyl-ACP TE or a chimericacyl-ACP TE; a host cell or organism comprising the nucleic acid; a mutant acyl-ACP TE or chimericacyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE; and a method of altering the level of activity of a plant acyl-ACP TE.

Materials and methods for using an acyl&#x2014;acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis

The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL),pyruvate decarboxylase (PDC), andaldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1&#x3b1; subunit of pPDH, the E1&#x3b2; subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1&#x3b1; pPDH, E1&#x3b2; pPDH, E2 pPDH, mtPDH or ALDH.

Materials and methods for the alteration of enzyme and acetyl CoA levels in plants

The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1 subunit of pPDH, the E1 subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyurvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1 pPDH, E1 pPDH, E2 pPDH, mtPDH or ALDH.

The present invention relates to cuticular lipid genes, their gene products, and methods of use thereof, for the generation of new plant varieties having novel environmental, disease and pest resistance. In particular, certain isolated or enriched nucleic acids specifying cuticular lipid genes, or portions thereof, are disclosed.

Isolation and use of cuticular lipid genes

Growing community of inventors

Ames, IA, United States of America

Basil J Nikolau