The General Hospital Corporation

Massachusetts Institute of Technology

Basak Elif Uygun

Martin L Yarmush

Maria-Louisa Izamis

Mehmet Toner

Mustafa Korkut Uygun

Robert Marius Bieganski

Osman Berk Usta

Timothy Antonie Berendsen

Sinem Perk

Alejandro Soto-Gutierrez

Jack Milwid

Korkut Uygun

Herman Tolboom

Hiroshi Yagi

Carley Shulman

The present invention generally relates to methods and compositions to determine viability of an organ for transplantation and other medical purposes. One aspect of the invention relates to a method for assessing the viability of an organ by measuring the energy parameters to determine the energy level of the organ by determining the stored cellular energy (e.g., ATP levels), and/or energy consumption over a particular time period of viability. The energy parameters can be compared to reference energy parameters as a highly accurate and reliable prediction of viable cell yield, and organ viability. Another aspect of the invention relates methods to preserve or extend the time period of viability of an organ any combination of (i) preservation perfusion of the organ to prevent ischemic damage, (ii) chemical metabolic suppression of the organ e.g., using metabolic suppressants, (iii) metabolic suppression by physical or environmental conditions, e.g., sub-zero non-freezing storage.

Methods and compositions for preserving tissues and organs

One aspect of the invention provides a method for harvesting adult cells from an organ. The method includes perfusing the organ with a perfusate and isolating adult cells from the organ, thereby harvesting the adult cells from the organ. Another aspect of the invention provides a method for rehabilitating an organ. The method includes: dividing the organ into a first portion and a second portion, perfusing the first portion with a decellularization medium, isolating adult cells from the second portion, and recellularizing the first portion with a suspension of the adult cells, thereby rehabilitating the organ.

Isolated adult cells, artificial organs, rehabilitated organs, research tools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same

Growing community of inventors

Cambridge, MA, United States of America

Basak Elif Uygun