New England Biolabs, Inc.

The General Hospital Corporation

Barton E Slatko

Thomas C Evans, Jr

Chudi Guan

Lixin Chen

Tayyaba Hasan

Romualdas Vaisvila

Rebecca B Kucera

Ulysses W Sallum

Romaldas Vaisvila

Lauren E Sears

Tricia Lennox

Jeremy Foster

Photoactivatable pesticide compounds and methods for the use thereof in the elimination and detection of pests are provided.

&#x3b2;-lactamase targeted photosensitizer for pesticide and pest detection

Methods and compositions are provided for repairing a polynucleotide so that it can be copied with improved fidelity and/or yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a ligase and a cofactor selected from NADor ATP and incubating the polynucleotide with the reaction mixture in the absence of Endonuclease VI. The reaction mixture may further contain an AP endonuclease and a polymerase. If used, these enzymes may be selected according to their ability to withstand high temperatures. For example, the reaction mixture may be used prior to a polynucleotide synthesis reaction in which case enzymes that are not thermophilic may be used. The repair reaction is not time sensitive with respect to seconds, minutes or hours of incubation in the enzyme mixture in as much as the repair is effected rapidly and prolonged incubation is not generally adverse.

Repair of nucleic acids for improved amplification

Methods and compositions are provided for repairing a polynucleotide so that it can be synthesized efficiently with improved fidelity and yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a ligase and a cofactor selected from NAD+ or ATP and incubating the polynucleotide with the reaction mixture in the absence of Endonuclease VI. The reaction mixture may further contain an AP endonuclease and a polymerase. These enzymes are optionally selected according to their ability to withstand high temperatures so they can be included in an amplification mixture. The reaction mixture may be used prior to a polynucleotide synthesis reaction in which case enzymes that are not thermophilic may be used. The repair reaction is not time sensitive with respect to seconds, minutes or hours of incubation in the enzyme mixture.

Purified thermostable inorganic pyprophosphatase obtainable from

Growing community of inventors

Ipswich, MA, United States of America

Barton E Slatko