Flodesign Sonics, Inc.

Flodesign, Inc.

Fiodesign Sonics, Inc.

Kedar C. Chitale

Jr., Walter M. Presz

Bart Lipkens

Benjamin Ross-johnsrud

Rui Tostoes

Jack Saloio

Walter M Presz, Jr

Jason Dionne

Thomas J Kennedy, Iii

Kedar C Chitale

Benjamin Ross-Johnsrud

Brian Dutra

Dane Mealey

Louis Masi

Brian McCarthy

Rudolf Gilmanshin

Stanley J Kowalski, Iii

Edward Rietman

Jason Barnes

Ben Ross-Johnsrud

Ari Mercado

Erik Miller

Jeffrey King

Arthur Martin

Ronald Musiak

Goutam Ghoshal

Ali Shajii

David Sokolowski

John Artis

Chris Leidel

Michael J Werle

Thomas Joseph Kennedy

Daniel Kennedy

Natalia Rodionova

Joey Carmichael

Eric Mitchell

Jeff King

Evgenia Zabolotskaya

Lauren Winiarski

John Rozembersky

Kedar Chitale

Iii Kennedy Thomas J

Jr Presz Walter M

Nicholas M LoRicco

Thomas P Kennedy

Michael Rust

Jason Gawencki

Anthony E English

Anthony B Masi

Richard Grant

Christopher Jurkiewicz

Stanley Kowalski

Edward A Rietman

Krishna N Kumar

Mark Brower

Lauryn Winiarski

Yuril Ilinskii

Yurii Ilinskii

Brian T Kennedy

Ruan Zhang

Brian Mccarthy

Iii Thomas J Kennedy

Jr Walter M Presz

Stanley Kowalski Iii

Thomas Kennedy Iii

Thomas J Iii Kennedy

Jr Walter Presz

Iii Stanley Kowalski

Iii Kowalski Stanley

J Kennedy Thomas

Walter M Jr

Krishna Kumar

Walter Presz

Walter M Presz

Beads with functionalized material applied to them are exposed to an acoustic field to trap, retain or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes. The acoustic field may be generated, for example, in an acoustic angled wave device or in an acoustic fluidized bed.

Acoustic cell separation techniques and processes

Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.

Expanded bed affinity selection

Beads with functionalized material applied to them are exposed to an acoustic field to trap or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes.

Affinity cell extraction by acoustics

An acoustophoretic system is controlled and driven to attain a desired level of performance. An RF controller and a driver provide a frequency and power to an acoustic transducer, which can be implemented as a piezoelectric element, which presents a reactive load or a complex load. A controller implements a control technique for efficient transducer operation. The control technique can locate a frequency for operation that is at a reactance minimum or maximum for the system to produce a modal pattern and to provide efficient operation of the transducer. A method of detecting a minimum or maximum reactance in a acoustophoretic system used to trap, separate, deflect, cluster, fractionate or otherwise process particles or secondary fluids or tertiary fluids in a primary fluid and utilizing the frequency of the detected reactance to operate the acoustophoretic system.

Acoustic transducer driver and controller

A closed and modular fluidic system composed of one or more acoustic elements and cell processing reagents. The system employs a cellular manufacturing process for producing cell and gene therapy therapeutics.

Cell therapy processes utilizing acoustophoresis

Acoustophoretic devices are disclosed. The devices include a flow chamber, an ultrasonic transducer, a reflector, an inlet, a filtrate outlet, a concentrate outlet, and optionally a lipid collection trap. The ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave in the flow chamber that traps and separates red blood cells and/or lipids from blood. Concentrated red blood cells can be recovered via the concentrate outlet, the lipids can be recovered via the lipid collection trap, and the remaining blood can be recovered via the filtrate outlet. Methods for separating blood components (e.g., red blood cells, lipids, platelets, white blood cells) from blood are also disclosed. The red blood cells can undergo washing with a solvent to remove undesired admixtures. Cryoprotectants can be added or removed from the blood.

Acoustic blood separation processes and devices

Multi-stage acoustophoretic devices for continuously separating a second fluid or a particulate from a host fluid are disclosed. Methods of operating the multi-stage acoustophoretic devices are also disclosed. The systems may include multiple acoustophoretic devices fluidly connected to one another in series, each acoustophoretic device comprising a flow chamber, an ultrasonic transducer capable of creating a multi-dimensional acoustic standing wave, and a reflector. The systems can further include pumps and flowmeters.

Concentration and washing of particles with acoustics

An acoustophoretic device is disclosed. The acoustophoretic device includes an acoustic chamber, an ultrasonic transducer, and a reflector. The ultrasonic transducer includes a piezoelectric material driven by a voltage signal to create a multi-dimensional acoustic standing wave in the acoustic chamber emanating from a non-planar face of the piezoelectric material. A method for separating a second fluid or a particulate from a host fluid is also disclosed. The method includes flowing the mixture through an acoustophoretic device. A voltage signal is sent to drive the ultrasonic transducer to create the multi-dimensional acoustic standing wave in the acoustic chamber such that the second fluid or particulate is continuously trapped in the standing wave, and then agglomerates, aggregates, clumps, or coalesces together, and subsequently rises or settles out of the host fluid due to buoyancy or gravity forces, and exits the acoustic chamber.

Non-planar and non-symmetrical piezoelectric crystals and reflectors

Therapeutic cell washing, concentration, and separation utilizing acoustophoresis

Methods and systems for separating material from a host fluid use an acoustophoresis device. These methods and systems can deflect material (e.g., a second fluid, cells, beads or other particles, exosomes, viruses, oil droplets) in host fluid streams at high flow rates.

Separation using angled acoustic waves

Aspects of the disclosure are directed to an apparatus for separating a second fluid or a particulate from a host fluid. That apparatus comprises a flow chamber with at least one inlet and at least one outlet. A drive circuit configured to provide a drive signal to a filter circuit configured to receive the drive signal and provide a translated drive signal. An ultrasonic transducer is cooperatively arranged with the flow chamber, and transducer includes at least one piezoelectric element configured to be driven by the current drive signal to create an acoustic standing wave in the flow chamber. At least one reflector opposing the ultrasonic transducer to reflect acoustic energy.

Electronic configuration and control for acoustic standing wave generation

An acoustophoresis device includes an acoustic chamber with a piezoelectric element located within its volume. The piezoelectric element vibrates and generates acoustic standing waves from both sides, so that particles can be separated from fluid passing through the acoustic chamber. This permits the element to be cooled more efficiently, reducing transient heat loads in the fluid traveling through the device.

Acoustophoresis device with dual acoustophoretic chamber

An acoustic standing wave is utilized to separate components from a multi-component fluid, such as oil from an oil-water mixture, or cells entrained in a fluid, in a fluid flow scheme with an acoustophoresis device. For example, the flow scheme and device allows for trapping of the oil as the oil coalesces, agglomerates, and becomes more buoyant than the water. A driver and controller for the acoustophoretic device accommodate variable loading as the components are separated, thereby improving separation efficiency.

Driver and control for variable impedence load

An apparatus for separating particles from a fluid stream includes a flow chamber that has at least one inlet and at least one outlet. At least one ultrasonic transducer is located on a wall of the flow chamber. The transducer includes a piezoelectric array with at least two piezoelectric elements. The piezoelectric array includes a piezoelectric material to create a multi-dimensional acoustic standing wave in the flow chamber. A reflector is located on the wall on the opposite side of the flow chamber from the at least one ultrasonic transducer.

Acoustophoretic device with piezoelectric transducer array

Growing community of inventors

Hampden, MA, United States of America

Bart Lipkens