Dow Agrosciences LLC

Agrigenetics, Inc.

Barry W Schafer

Lining Guo

Scott A Young

Rod Aaron Herman

Ignacio Mario Larrinua

Scott Bevan

Jon Christopher Mitchell

Scott B Bintrim

Patricia C Apel-Birkhold

Susan B Green

Donald J Merlo

Ann Owens Merlo

James K Petell

Jean L Roberts

Kitisri Sukhapinda

Dave R Albers

Krishna Kuppannan

Samir Julka

Embodiments of the invention include methods of determining the allergen content of a composition. Embodiments of the invention may include providing a composition comprising an allergen; at least partially purifying the allergen from the composition to form an extract; and determining the amount of allergen in the extract using liquid chromatography with ultraviolet and mass spectrometric detection.

Quantification and characterization of allergens

The invention provides a method for devitalizing plant seed, the method comprising the steps of hydrating a viable whole plant seed and freezing the hydrated whole plant seed. The invention further provides a collection of devitalized whole plant seed wherein the integrity of genomic DNA and protein within the devitalized plant seed is preserved.

Method for seed devitalization

The subject invention relates to noveltoxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable fromstrain Xwi. The subject invention also provides an exochitinase obtainable from the Xwi strain. This exochitinase can be used to control insects using methods known in the art.

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The subject invention relates to novel nucleic acid encoding astrain Xwi toxin complex (TC) protein and plants and bacteria transformed therewith.

Novel polynucleotide sequences that encode insect toxins TcdA and TcbA have base compositions that differ substantially from the native genes, making them more similar to plant genes. The new sequences are suitable for use for high expression in both monocots and dicots. Transgenic plants with a genome comprising the nucleic acid of SEQ ID NO:4 are insect resistant.

Transgenic plants expressing photorhabdus toxin

Novel polynucleotide sequences that encode insect toxins TcdA and TcbA have base compositions that differ substantially from the native genes, making them more similar to plant genes. The new sequences are suitable for use for high expression in both monocots and dicots. Transgenic plants with a genome comprising a nucleic acid of SEQ ID NO: 3 or SEQ ID NO:4 are insect resistant.

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Cicero, IN, United States of America

Barry W Schafer