Miles Laboratories, Inc.

Miles Inc.

Barry S Leng

Raleigh A Carmen

Chiyong Chong

Edward J Nelson

Willie J Lewis

Carmen, Raleigh A

Randy B Garcez

A method of preparing neocytes and gerocytes in a closed blood bag system useful for the filtration of leukocytes from a red blood cell concentrate followed by the preparation and long term storage of neocytes and, preferably, gerocytes from the filtered concentrate. In very preferred embodiments, the system also provides containers for the expression of plasma and the preparation and long term storage of platelets.

Method of preparing neocytes and gerocytes in a closed system

A generally flat, elongated edge-sealed polymeric blood bag having a length to width ratio of at least 2 to 1 and one end portion in tapering communication with a connected tubing. When the bag is filled with a mixture of blood components which are then separated, the tapering end portion expands to form a funnel-like guide for directing a separated component from the bag through the tubing in a substantially unobstructed manner. Bag is especially useful for separating and isolating the components of a neocyte/gerocyte red blood cell mixture.

Bag for separation and isolation of blood components

Closed filtering system for relatively fast and efficient removal of white blood cells from a red blood cell mixture. System comprises at least two blood bags in closed communication with each other via an intermediate filter assembly comprising a housing containing continuous filtering fiber. A preferred housing is tapered and the fiber preferably has a generally Y-shaped cross sectional area and it is adapted to permit substantial removal of white blood cells from a red blood cell mixture with minimal red blood cell hemolysis when the mixture is diluted with preservative solution and passed from one bag to the other at a relatively high flow rate. Filtration is completed within 24 hours, preferably within 6 hours, of whole blood donation.

Red blood cell filtering system

Closed filtering system for relatively fast and efficient removal of white blood cells from a red blood cell mixture. System comprises at least two blood bags in closed communication with each other via an intermediate filter assembly comprising a housing containing continuous filtering fiber. A preferred housing is tapered and the fiber preferably has a generally Y-shaped cross sectional area and it is adapted to permit substantial removal of white blood cells from a red blood cell mixture with minimal red blood cell hemolysis when the mixture is diluted with perservative solution and passed from one bag to the other at a relatively high flow rate. Filtration is completed within 24 hours, preferably within 6 hours, of whole blood donation.

Aqueous storage solution for red blood cells comprising a stabilized ascorbate derivative and having a pH sufficient to maintain substantially all of the initial concentration of ascorbate derivative and avoid formation of ascorbate degradation products during heat sterilization. A preferred solution includes at least about 90% of the pre-sterilization ascorbate and adenine but is free of dextrose.

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Pleasant Hill, CA, United States of America

Barry S Leng