Wescor Inc.

Other

Barry O Stokes

Carmelo G Quirante

Gary D Bradshaw

Patti A Nelson

Wayne K Barlow

John Gibson

Kevin M Mullen

Dennis R Briscoe

Randal P Holman

Marilyn L Grover

A cytocentrifuge sample chamber includes a sample receiving well to receive and hold a sample to be centrifuged and a sedimentation chamber adjacent a microscope slide upon which cells and/or other sediment material in the sample is to be deposited. A passage, such as a spillway, connects the sample receiving well and the sedimentation chamber, so that sample flows from the sample well under the influence of centrifugal force as centifugation begins into the sedimentation chamber where the sample is forced by centrifugal force against the microscope slide leaving a vacant space at the inner end of the sedimentation chamber. Sedimentation of the sample takes place against the microscope slide. When centrifugation ends, the liquid components of the sample flow back into the vacant space in the sedimentation chamber and can be removed from the vacant space at the inner end of the sedimentation chamber through a removal chamber. This avoids contamination of equipment and users and allows a secondary centrifugation to take place.

Large area cytocentrifuge sample chamber

Automated staining equipment that can mix reagents is used to spray a Romanowsky stain onto slide mounted specimens which are then briefly centrifuged. The centrifugation step removes excess stain leaving only a thin film. Depending on the time of the centrifugation step, most of the organic solvent and part of the water in the stain are evaporated by airflow through the equipment. This greatly accelerates the staining reaction and preserves water soluble structures such as the granules in basophilic leukocytes. For optimal performance, this staining procedure requires a thiazin-eosin stain with about 90% to about 40% organic solvent, such as methanol, and only about 10% to about 60% water. This is a unique staining reagent in Romanowsky staining.

Staining reagent for staining hematology samples in an automated staining apparatus

Method and staining reagent for staining hematology sample in an automated staining apparatus

In accordance with the apparatus of the invention, a preferably vertically upstanding housing is provided, internally thereof, with a rack structure having an upstanding series of shelves, or other microscope-slide-supporting members, spaced one above another providing multiple, substantially horizontal slots arranged alternatively with the shelves and leading into corresponding compartments between the shelves for receiving microscope slides to be heated, which preferably lie flatwise on and against the shelves so as to be heated by conduction from the shelves. The method of the invention constitutes the placing of the slides in the heating compartments, preferably with corresponding ends projecting therefrom so as to remain relatively cool for grasping during removal, and turning on the heat.

Apparatus and method for heating microscope slides

A cytocentrifugation device, rotor, and apparatus are improved by providing the device with a plurality of liquid-receiving chambers arranged serially along and opening into an elongate conduit leading to and terminating within a filter-pad-holder so that a filter-pad-prewetting liquid can be passed along such conduit and into a liquid-flow opening of a filter pad held by such holder in advance of passage along said conduit and through such liquid-flow opening in the filter pad of a cell-carrying liquid sample during a centrifugation run of the apparatus. Retention of prewetting liquid in the filter pad around the sample liquid flow helps to prevent loss of cells to the filter pad. Various other structural modifications of the cytocentrifugation device and filter pads used therewith also help to prevent loss of cells to the filter pad.

Liquid absorption pad for cytocentrifugation device

Cytocentrifuge rotor for cytocentrifugation devices

In the field of staining methods for histology and cytology specimens, the improvements of the invention are three-fold. First included is a drying-clearing step whereby a specimen is cleared by drying. Thus, the use of hazardous chemical-clearing agents to remove washing-dehydrating solution prior to cover-slipping is eliminated. Second, in a staining method using spray staining equipment, the use of a low molarity, combination, bluing-washing solution which serves the dual purpose of a washing solution and a bluing reagent, eliminates the use of water in a staining procedure. Third, and also in a staining method using spray staining equipment, the use of a series of sprays of fresh, essentially anhydrous, washing-dehydrating solution to dehydrate a specimen replaces immersion in a series of washing-dehydrating solutions and thereby decreases the amount of chemical consumed and also avoids contamination of the reagent with water and specimen debris.

Staining methods for histology and cytology specimens

Cytocentrifugation apparatus is improved by providing centrifuging devices with a plurality of liquid-receiving chambers arranged serially along and opening into an elongate conduit leading to and terminating within a filter-pad-holder so that a filter-pad-prewetting liquid can be passed along such conduit and into a liquid-flow opening of a filter pad held by such holder in advance of passage along said conduit and through such liquid-flow opening in the filter pad of a cell-carrying liquid sample during a centrifugation run of the apparatus. Retention of prewetting liquid in the filter pad around the sample liquid flow helps to prevent loss of cells to the filter pad. Various other structural modifications of the cytocentrifugation device and filter pads used therewith also help to prevent loss of cells to the filter pad.

Cytocentrifugation device, apparatus, and method

Apparatus for applying a controlled amount of reagent to one or more miscroscope slide or the like includes a spray chamber and a carousel adapted to hold a plurality of slides and to be mounted for rotation in the spray chamber. Nozzles are mounted to spray reagent onto the slides in the carousel as they rotate by the nozzles. Accurate control of the time or angular rotation during which the reagent is sprayed from the nozzles is obtained by placing a control valve in the flow line between a reagent pump and the nozzle and operating the valve to control flow of reagent. Control circuitry determines the position of the slides and carousel in the spray chamber and coordinates operation of the pumps, valves, and rotation of the carousel to accurately cause spray of reagent onto the slides. The slide holding carousel may be replaced by an interchangable centrifuge rotor when it is desired to perform cytocentrifuging tests.

Apparatus for applying a controlled amount of reagent to a microscope

A fixative solution for fixing biological smears, such as blood smears, to a slide for subsequent staining of the smear, which can be used in automated slide staining equipment, includes a fixative liquid, a stabilizing agent to reduce water spotting problems during fixing and/or to stabilize cellular components of the smear, and a solubilizing agent for maintaining the stabilizing agent in solution in the solubilizing agent after evaporation of the fixative liquid to thereby prevent formation of the solid residue normally formed by the stabilizing agent upon evaporation of the fixative liquid. The invention also includes the method fo preventing formation of a solid residue upon evaporation of the fixative liquid from a fixative liquid-stabilizing agent solution by the addition of a solubilizing agent thereto. The mixture of the stabilizing agent and solubilizing agent can be made as a concentrated additive to be added to a fixative liquid prior to use. The preferred stabilizing agent is polyvinyl pyrrolidone and the preferred solubilizing agent is ethylene glycol.

Fixative composition for fixing blood smears to slides

Apparatus for applying a controlled amount of reagent to a microscope slide or the like includes a spray chamber and a carousel adapted to hold a plurality of slides and to be mounted for rotation in the spray chamber. Nozzles are mounted to spray reagent onto the slides in the carousel as they rotate by the nozzles. Accurate control of the time during which the reagent is sprayed from the nozzles is obtained by placing a control valve in the flow line between a reagent pump and the nozzle and operating the valve to control flow of reagent. Control circuitry coordinates operation of the pumps, valves, and rotation of the carousel to accurately cause spray of reagent onto the slides.

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Logan, UT, United States of America

Barry O Stokes