Hercules Corporation

Franhofer USA Incorporated

Fraunhofer USA, Inc.

Barry Marrs

Simon Delagrave

James A Romesser

Carla H Kuhner

James Romesser

A method of interfering with quorum sensing regulation of genes to promote cell growth is disclosed. The method of is aimed at accessing microbial biodiversity. The method involves obtaining an environmental sample comprising at least one novel (uncultivated in the laboratory) microorganism, contacting the environmental sample with an effective amount of an agent or combination of agents which interferes with the quorum sensing regulation of genes, growing the treated sample in a culture medium containing the quorum sensing signal disrupting agent or agents, and analyzing the colonies of microorganisms grown to demonstrate genetic novelty.

Method for accessing microbial diversity

The present invention provides methods of preparing large polynucleotides of arbitrary sequence and in a manner that will readily lend itself to automation. The present invention provides methods of preparing a polynucleotide having at least 200 nucleotides in either a 5&prime; to 3&prime; or 3&prime; to 5&prime; direction by ligating a plurality of oligonucleotides, the assembly of which, represents the nucleotide sequence of the desired polynucleotide. The present invention also provides libraries of polynucleotides and screening of libraries for polynucleotide members having desired properties.

Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics

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Kennett Square, PA, United States of America

Barry Marrs