Binx Health Limited

Atlas Genetics Limited

Barrie J Marsh

Christopher G Frost

David M Pearce

Jonathan Sharp

Stephen Flower

Jonathan C Sharp

Compounds of general formula I wherein Fc and Fc′ may be the same or different and are substituted ferrocenyl moieties having at least one ring substituent selected from sulfur-containing groups, phosphorus-containing groups, iodo, chloro, silyl, fluoroalkyl groups containing two or more fluorine atoms, heteroaryl, substituted phenyl, and cyano, wherein if present as sole substituent the cyano group is located on the proximal cyclopentadienyl ring; X is a spacer, Y is a spacer, Z is a spacer; and R is a linker group. Compound I may be used to make labelled substrates, functionalised compounds for making labelled substrates and may be used as labels in an electrochemical assay.

1,1'-[(substituted alkyl)imino]bis(alkylene)]bis-ferrocenes and their use in electrochemical assays by labelling substrates of interest

Compounds of general formula I wherein Fc and Fc' may be the same or different and are substituted ferrocenyl moieties having at least one ring substituent selected from sulfur-containing groups, phosphorus-containing groups, iodo, chloro, silyl, fluoroalkyl groups containing two or more fluorine atoms, heteroaryl, substituted phenyl, and cyano, wherein if present as sole substituent the cyano group is located on the proximal cyclopentadienyl ring; X is a spacer, Y is a spacer, Z is a spacer; and R is a linker group. Compound I may be used to make labelled substrates, functionalised compounds for making labelled substrates and may be used as labels in an electrochemical assay.

1,1 '-[[(substituted alkyl)imino]bis (alkylene)]bis- ferrocenes and their use in I electrochemical assays by labelling substrates of interest

Compounds of general formula I are used as labels in an electrochemical assay: (I) in which: Fc and Fc' are substituted or unsubstituted ferrocenyl moieties, X is a C1 to C6 alkylene chain which is optionally interrupted by &#x2014;O&#x2014; or &#x2014;NH&#x2014;; Y is a C1 to C6 alkylene chain which is optionally interrupted by &#x2014;O&#x2014; or &#x2014;NH&#x2014;; Z is a C1 to C12 alkylene chain which may optionally be substituted and/or may optionally be interrupted by &#x2014;O&#x2014;, &#x2014;S&#x2014;, cycloalkyl, &#x2014;CO&#x2014;, &#x2014;CONR1-, &#x2014;NR1CO&#x2014; or &#x2014;NR1- in which R1 represents hydrogen or C1 to C4 alkyl; and R is a linker group. Compounds I are used to make labelled substrates, as well as functionalised compounds for making the labelled substrates.

Ferrocene labels for electrochemical assay and their use in analytical methods

The invention provides monoferrocenyl compounds of general formula I. The invention also provides substrates labelled with the compounds, functionalised derivatives of the compounds and methods of using the compounds, functionalised derivatives and labelled substrates in electrochemical assays.

Labelling compounds and their use in assays

Compounds of general formula I wherein Fc and Fc' may be the same or different and are substituted ferrocenyl moieties having at least one ring substituent selected from sulfur-containing groups, phosphorus-containing groups, iodo, chloro, silyl, fluoroalkyl groups containing two or more fluorine atoms, heteroaryl, substituted phenyl, and cyano, wherein if present as sole substituent the cyano group is located on the proximal cyclopentadienyl ring; X is a spacer, Y is a spacer, Z is a spacer; and R is a linker group. Compound I may be used to make labelled substrates, functionalised compounds for making labelled substrates and may be used as labels in an electrochemical assay. 

1,1 '-[[(substituted alkyl)imino]bis(alkylene)]bis- ferrocenes and their use in I electrochemical assays by labelling substrates of interest

Compounds of general formula I are used as labels in an electrochemical assay: (I) in which: Fc and Fc' are substituted or unsubstituted ferrocenyl moieties, X is a C1 to C6 alkylene chain which is optionally interrupted by &#x2014;O&#x2014; or &#x2014;NH&#x2014;; Y is a C1 to C6 alkylene chain which is optionally interrupted by &#x2014;O&#x2014; or &#x2014;NH&#x2014;; Z is a C1 to C12 alkylene chain which may optionally be substituted and/or may optionally be interrupted by &#x2014;O&#x2014;, &#x2014;S&#x2014;, cycloalkyl, &#x2014;CO&#x2014;, &#x2014;CON R1&#x2014;, &#x2014;NR1CO&#x2014; or &#x2014;NR1&#x2014; in which R1 represents hydrogen or C1 to C4 alkyl; and R is a linker group. Compounds I are used to make labelled substrates, as well as functionalised compounds for making the labelled substrates.

1,1'-[[(substituted alkyl)imino]bis(alkylene)]bis-ferrocenes and their use in I electrochemical assays by labelling substrates of interest

Compounds of general formula I wherein Fc and Fc' may be the same or different and are substituted ferrocenyl moieties having at least one ring substituent selected from sulfur-containing groups, phosphorus-containing groups, iodo, chloro, silyl, fluoroalkyl groups containing two or more fluorine atoms, heteroaryl, substituted phenyl, and cyano, wherein if present as sole substituent the cyano group is located on the proximal cyclopentadienyl ring; X is a spacer. Y is a spacer, Z is a spacer; and R is a linker group. Compound I may be used to make labelled substrates, functionalised compounds for making labelled substrates and may be used as labels in an electrochemical assay. 

1,1 '-[[(Substituted alkyl)imino]bis(alkylene)]bis-ferrocenes and their use in I electrochemical assays by labelling substrates of interest

The invention provides monoferrocenyl compounds of general formula (I). The invention also provides substrates labelled with the compounds, functionalised derivatives of the compounds and methods of using the compounds, functionalised derivatives and labelled substrates in electrochemical assays.

Growing community of inventors

Trowbridge, United Kingdom

Barrie J Marsh