Flagship Pioneering Innovations Vi, LLC

Editas Medicine, Inc.

Barrett Ethan Steinberg

Hariharan Jayaram

Robert James Citorik

Anne Helen Bothmer

William Edward Salomon

Robert Charles Altshuler

Randi Michelle Kotlar

Nathaniel Roquet

Cecilia Giovanna Silvia Cotta-Ramusino

Ananya Ray

Jacob Rosenblum Rubens

Ari E Friedland

Carlos Sanchez

Zi Jun Wang

Derek Cerchione

Kyusik Kim

Geoffrey Von Maltzahn

Sandeep Kumar

Stephen Hoyt Cleaver

Donghui Li

Luciano Henrique Apponi

Joshua C Tycko

Nick Huston

Daniel Raymond Chee

Aamir Mir

Gregory David McAllister

Zhan Wang

William Querbes

Methods and compositions for modulating a target genome are disclosed. This disclosure relates to novel compositions, systems and methods for altering a genome at one or more locations in a host cell, tissue or subject, in vivo or in vitro. In particular, the invention features compositions, systems and methods for inserting, altering, or deleting sequences of interest in a host genome.

Methods and compositions for modulating a genome

Methods and compositions for modulating a target genome are disclosed. The composition may comprise a first RNA encoding a polypeptide comprising a retrotransposase reverse transcriptase domain and a retrotransposase endonuclease domain. The composition may also comprise a second RNA comprising a sequence that binds the polypeptide and a heterologous object sequence. The composition may insert the sequence of the heterologous object sequence into a target DNA.

The present disclosure relates to Cas9 nuclease variants and methods of producing and using such variants.

Engineered Cas9 nucleases

The present disclosure relates to systems and methods of analyzing cleavage profiles of nucleases.

Assessing nuclease cleavage

The disclosure provides, e.g., compositions, systems, and methods for targeting, editing, modifying, or manipulating a host cell's genome at one or more locations in a DNA sequence in a cell, tissue, or subject. Gene modifying systems for treating cystic fibrosis, e.g., in subjects having a mutation resulting in F508del, are described.

CFTR-modulating compositions and methods

Engineered nucleic acids encoding genome editing system components are provided, as are engineered RNA-guided nucleases that include inserts encoded in part by cellular genomic or other sequences recognized by guide RNAs.

Systems and methods for one-shot guide RNA (ogRNA) targeting of endogenous and source DNA

The disclosure provides, e.g., compositions, systems, and methods for targeting, editing, modifying, or manipulating a host cell's genome at one or more locations in a DNA sequence in a cell, tissue, or subject.

Methods and compositions for modulating a target genome are disclosed.

Growing community of inventors

Somerville, MA, United States of America

Barrett Ethan Steinberg