Life Technologies Corporation

Applera Corporation

Applied Biosystems, LLC

The Perkin-elmer Corporation

Pe Corporation (ny)

Other

Lift Technologies Corporation

The Perlein-elmer Corporation

Barnett B Rosenblum

Linda G Lee

Steven Menchen

Shaheer Khan

Sandra Spurgeon

Marian Peris

Michael Phelan

Scott C Benson

Theo Nikiforov

Meng Taing

Peter B Vander Horn

Sandra L Spurgeon

Michael Previte

Cheng-Yao Chen

Guobin Luo

Hongye Sun

Yufang Wang

Zhaohui Zhou

Weiguo Zhen

Shaheer H Khan

Jamshid Temirov

Hongyi Wang

Ronald J Graham

Khairuzzaman Bashar Mullah

Stephen P Hendricks

David Light

Daqing Sun

Joe Y L Lam

Paul M Kenney

Jonathan M Cassel

Wolfgang Hinz

Earl A Hubbell

Carl Williams Fuller

Dmitriy Gremyachinskiy

Alexander J Mastroianni

Stefanie Yukiko Nishimura

Geun-sook Jeon

Zhaochun Ma

Luisa Andruzzi

Ravi S Vinayak

Christian Woehler

Jer-Kang Chen

Roman Rozhkov

Joe Y Lam

Craig Stolarczyk

Diem Tran

Tanya Sokolsky

Boli Huang

Shoeb Khan

Geir Fonnum

Jo Aaserud

Nini Kjus

Astrid Evenroed Molteberg

Grete Modahl

M Talha Gokmen

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

Nucleotide transient binding for sequencing methods

The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.

Thiolated nucleotide analogues for nucleic acid synthesis

In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.

Methods of nucleic acid analysis using terminator nucleotides

Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

Mutant RB69 DNA polymerase

The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.

Polymer particles, nucleic acid polymer particles and methods of making and using the same

Disclosed are methods and kits applicable to sequencing methods, such as Sanger dideoxy sequencing methods. The methods and kits disclosed utilize a cationically charged nucleic acid terminator in combination with a discriminatory polymerase.

Nucleic acid terminators incorporating a cationic moiety and methods for their use

Growing community of inventors

San Jose, CA, United States of America

Barnett B Rosenblum