The United States of America, as Represented by the Secretary, Department of Health and Human Services

Dana-Farber-Cancer Institute Inc.

Barna Dey

Gary J Nabel

Peter D Kwong

Joseph G Sodroski

Tongqing Zhou

Ling Xu

Wen Yuan

Steven A Rosenberg

Richard A Morgan

John R Mascola

Li Liu

Richard Wyatt

Shi-Hua Xiang

Edward A Berger

Bhavik Patel

Mustafa H Ghanem

Chih-Chin Huang

The present disclosure is directed to novel multispecific chimeric antigen receptor (CAR) proteins and DNA sequences encoding these proteins. The CARs comprise at least two extracellular domains fused, via a transmembrane domain to a cytoplasmic signaling domain comprising two signaling domains. The disclosure further relates to nucleic acids encoding the novel CARs, to host cells expressing the novel CARs, and to methods of using the CARs to co-stimulate effector functions in the cells and for using cells expressing the receptors for treatment of disease and viral infections. The disclosure also relates to methods of generating a recombinant T cell with reduced susceptibility to HIV infection.

Chimeric antigen receptors to control HIV infection

Isolated immunogens including a HIV-1 gp120 polypeptide or immunogenic fragment thereof stabilized in a CD4 bound confirmation by crosslinked cysteines, and methods of their use are disclosed. The immunogens are useful, for example, for generating an immune response to HIV-1 gp120 in a subject.

Conformationally stabilized HIV envelope immunogens

Stabilized forms of gp120 polypeptide, nucleic acids encoding these stabilized forms, vectors comprising these nucleic acids, and methods of using these polypeptides, nucleic acids, vectors and host cells are disclosed. Crystal structures and computer systems including atomic coordinates for stabilized forms of gp120, and gp120 with an extended V3 loop, and methods of using these structures and computer systems are also disclosed.

Conformationally stabilized HIV envelope immunogens and triggering HIV-I envelope to reveal cryptic V3-loop epitopes

Growing community of inventors

Germantown, MD, United States of America

Barna Dey