Roche Molecular Systems, Inc.

Roche Sequencing Solutions, Inc.

Genia Technologies, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Barbara Eckert

Shawn Lee Suko

Aruna Ayer

Mara Boenitz-Dulat

Eileen Thai

Ilya Lederman

Charles Wayan Schwab

Colin Alexander McGaw

Cleoma Arnold

David Daniel Wunderlich

Bigna Woersdoerfer

Arkadiusz Bibillo

Tyler O'Brien Shultz

Preethi Sarvabhowman

Evan Amato

Keith Bauer

Kalyani Mangipudi

Kyle C Cady

Patrick Lin

Hai Nguyen

Colin Lam

Jeffrey Alexander

Kristina Chu

Dylan Goldman

Misha Mehta

Arrash Moghaddasi

Natacha Sorenson

Keith A Bauer

Shawn Suko

Cleoma Renetta Arnold

Tyler O'Brien Schultz

Disclosed are DNA polymerases having improved ability to incorporate methylated-dNTPs, relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases. Further disclosed are kits and reaction mixtures comprising the improved DNA polymerases as well as methods of primer extension using the improved DNA polymerases.

DNA polymerases for efficient and effective incorporation of methylated-dNTPS

The present invention relates to compositions comprising and methods of producing genetically engineered bacteriophages, bacteriophage-like particles and non-replicating transduction particles (NRTPs) that contain non-native tail fibers that display altered host specificity and/or reactivity. The present invention also relates to methods of using these bacteriophages and NRTPs for the development of novel diagnostics, therapeutics and/or research reagents for bacteria-related diseases.

Non-replicative transduction particles with one or more non-native tail fibers and transduction particle-based reporter systems

Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365Q, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, I644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.

Polymerase variants

The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.

Pol6 polymerase variants

Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365Q, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, 1644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.

Described herein are variant pol6 polymerase having at least having at least two substitutions, wherein at least one of the substitutions is at position S366 of SEQ ID NO: 2. The disclosed polymerases may be coupled to alpha-hemolysin nanopores, and may be used in nanopore-based sequencing apparatuses.

Growing community of inventors

El Cerrito, CA, United States of America

Barbara Eckert