Academy of National Food and Strategic Reserves Administration

Baoxia Ni

Hongmei Liu

Jinnan Chen

Songxue Wang

Jin Ye

Disclosed is a magnetic chemiluminescence immunoassay kit based on a bifunctional fusion protein for mycotoxins, and a use thereof. The kit includes streptavidin magnetic particles, a biotin-labeled mycotoxin antigen, a mycotoxin standard solution, a mycotoxin single-chain variable fragment (ScFV)-alkaline phosphatase bifunctional fusion protein, a sample diluent, a washing solution and a substrate solution. The kit of the disclosure integrates chemiluminescence, magnetic particle separation and gene engineering technologies, thereby quickly, accurately and automatically testing the mycotoxins; and edible oil can be directly added for test without operations of extraction, centrifugation, etc., thereby reducing operation errors of testing personnel, reducing capability requirements on the testing personnel, significantly improving testing efficiency of large quantities of samples at a grassroots level, and providing a powerful means for mycotoxin testing and monitoring.

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Beijing, China

Baoxia Ni