Shandong Institute for Food and Drug Control

Shandong University

Avanc Pharmaceutical Co., Ltd

Penglai Nuokang Pharmaceutical Co., Ltd.

Baojian Hang

Feng Shi

Lianli Chi

Ruiqing Xian

Liping Gong

Congcong Wang

Weijian Wang

Fengshan Wang

Hongming Du

Bin Zhang

Jing Dong

Mengmeng Li

Qunye Zhang

Xunjie Zhang

Li Nie

Pengfei You

Deling Shi

Honghai Li

The present invention provides a snake venom thrombin marker peptide ofand an application of the snake venom thrombin-like enzyme in identifying species of Hemocoagulase for Injection. The application includes the following steps of: dissolving a to-be-detected sample and a reference substance of the marker peptide respectively to prepare a test solution and a reference solution, and conducting alkylation reduction on the test solution and the reference solution with dithiothreitol and iodoacetamide; after diluting products with an ammonium bicarbonate solution, adding enzyme for hydrolysis; and after enzymolysis is finished, conducting centrifugation at a high speed, and injecting a supernatan into a liquid chromatography-mass spectrometer for analysis. This method is simple, convenient and rapid, is strong in specificity, fills the gap in identifying the source of species of the snake venom thrombin-like enzyme ofand improves the quality control level.

Snake venom thrombin-like enzyme marker peptide ofand its application in the species identification of hemocoagulase for injection

Disclosed are specifically a marker polypeptide of a-like thrombin and a method thereof for detecting species source and content of a snake venom-like thrombin and an application, relating to the technical field of snake venom detection. An amino acid sequence of the marker polypeptide is EAYNGLPAK (SEQ ID NO:1), and the marker polypeptide may be used to detect the species source and content of the snake venom-like thrombin in a sample. The marker polypeptide of the present disclosure may play an important role in characterizing the species source and content of the snake venom-like thrombin in the sample, and fill in the blank of a quality standard of snake venom of the

Marker polypeptide of bothrops atrox-like thrombin and method thereof for detecting species source and content of snake venom-like thrombin and application

The present application is related to a method for identifying whether porcine heparin is adulterated with heparin from ruminants, comprising: (1) respectively detecting the contents of trisaccharide(4S) and &#x394;UA2S-GlcNAc6S (&#x394;IA) in a sample and at least three batches of porcine heparin standards; (2) calculating a ratio of the trisaccharide(4S) to the &#x394;IA as well as a standard deviation (SD) of the ratio in the porcine heparin standards; when the ratio of the trisaccharide(4S) to the &#x394;IA in the sample exceeds a maximum value of the ratio in the porcine heparin standards+3SD, where the sample is considered to be mixed or adulterated with heparin from ruminants; wherein the detection method used is hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS) or multiple reaction monitoring (MRM). The method can distinguish porcine heparin from ovine and bovine heparin based on the structural differences, regardless of the production process the heparin has undergone.

Method for identifying whether porcine heparin is adulterated with heparin from ruminants

The present invention relates to the technical field of chemical analysis and quantitative detection, in particular to a quantitative detection method for snake venom thrombin-like enzyme (SVTLE) from. The quantitative detection method for the SVTLE includes the following steps of taking a reference substance of marker peptide for the SVTLE fromwith an amino acid sequence of LDSPVSNSAHIAPLSLPSSAPSVGSVCR, and preparing a series of reference solutions with different concentrations; adding the reference solutions in test solutions respectively for enzymolysis, and then taking a supernatant after enzymolysis as a series of solutions to be detected; and adding the solutions to be detected in a liquid chromatogram-mass spectrometer, and then selecting a qualitative ion pair and a quantitative ion pair to detect contents of marker peptide in the solutions to be detected.

Quantitative detection method for snake venom thrombin-like enzyme (SVTLE)

The present invention relates to the field of chemical analysis detection and application, in particular to a marker peptide of snake venom thrombin-like enzymes (SVTLEs) fromand an application thereof. The amino acid sequence of the marker peptide of snake venom thrombin-like enzymes (SVTLEs) fromis TLCAGVMEGGIDTCNR. Characterizing the source of species and a content of the SVTLEs in a to-be-detected sample by using the marker peptide includes the following steps of: pretreating the to-be-detected sample by trypsin through enzymolysis, and taking a supernatant of an enzymolysis liquid as a test solution; and injecting the test solution and a reference solution into a liquid chromatography-mass spectrometer, and selecting a qualitative ion pair and a quantitative ion pair for detecting the source of species and a content of the SVTLEs in the to-be-detected sample.

Marker peptide of snake venom thrombin-like enzymes (SVTLEs) from agkistrodon halys pallas and application thereof

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