Cleveland State University

Glc Biotechnology, Inc.

Baochuan Guo

Yiding Liu

Xiyuan Sun

Shenyi Wang

Provided herein is materials and method relating to nucleic acids extraction and purification from biological samples. In particular, a pre-treatment buffer is used to facilitate the extraction and purification of nucleic acids from biological samples, more specifically, removing inhibitors and impurities from biological samples and resulting in a highly concentrated and purified nucleic acids preparation.

Method and materials for nucleic acids extraction and purification

Provided herein is materials and method relating to nucleic acids extraction and purification from biological samples such as stool. In particular, binding protein(s) is used to facilitate the extraction and purification of nucleic acids from stool samples, more specifically, depleting and blocking inhibitors and impurities from stool samples and resulting in a highly concentrated and purified nucleic acids preparation.

Method and materials to deplete impurities for extraction and purification of nucleic acids from stool

Methods for simultaneously surveying the status of a large number of DNA mutation markers are described. In addition, methods for simultaneously determining the methylation status at multiple sites of a collection of genes, in a single assay, are described.

Methods for identifying multiple DNA alteration markers in a large background of wild-type DNA

The mass resolution and accuracy of delayed extraction matrix assisted desorption/ionization time of flight mass spectrometry is improved by spatially separating the electric field used for driving extraction/acceleration from the sample being analyzed.

SDIFA mass spectrometry

The VSET method comprises: providing a polynucleotide acid sample comprising at least one target site, and a first region of nucleotides immediately adjacent to the target site; preferably genomic DNA; preferably amplifying the polynucleotide; then combining the polynucleotide sample with: three dideoxynucletides selected from the group of ddGTP, ddATP, ddCTP, and ddTTP; and one deoxynucleotide selected from the group consisting of and dGTP, dATP, dCTP, and dTTP wherein the nucleotide of the deoxynucleotide is not the same as the nucleotide in the dideoxynulceotide; and a mini-sequencing primer complementary to the first region of nucleotides; extending the mini-sequencing primer with a dideoxynucletide or deoxynulceotide whose base is complementary to the base at the target site, to provide extension products; and then identifying the extension products, preferably by (MALDI-TOF) mass spectrometry. One of the three ddNTps is complementary to one of the allelic variations at the single point mutation site while the deoxynucleotide is complementary to the other allelic variant at the single point mutation site. The mini-sequencing primer hybridizes to a polynucleotide sequence immediately next to a single point mutation site and is extended by the DNA polymerases. The genotype at the variable site is determined on the basis of the number of nucleotides contained in the extension products.

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Solon, OH, United States of America

Baochuan Guo