Lanzatech New Zealand Limited

Lanzatech Nz, Inc.

Bakir Al-Sinawi

Christophe Collet

Michael Koepke

Sean Dennis Simpson

Richard Llewellyn Sydney Forster

Christophe Daniel Mihalcea

Jason Carl Bromley

Shilpa Nagaraju

Loan Phuong Tran

Matthew Rowe

Phuong Loan Tran

Will David Barker

Bjorn Daniel Heijstra

Jennifer Mon Yee Fung

Wing Chuen Chan

Gary Chan

Kelly Marie Mahar

Sashini De Tissera

Matthew James Rowe

Provided is a method of producing a product by culturing a carboxydotrophic acetogenic bacterium with a disrupting mutation in a lactate dehydrogenase enzyme in the presence of a substrate comprising CO, CO, and/or H. Preferably, the disrupting mutation reduces or eliminates the expression or activity of the enzyme such that the bacterium produces a reduced amount of lactate or no lactate.

Recombinant microorganisms and methods of use thereof

One or more genes in a biosynthesis pathway for a vitamin or other essential nutrient which is needed for the survival of a microorganism can be used as an effective selective marker to identify cells transformed with an exogenous nucleic acid. The microorganism does not naturally contain or express the one or more gene. This permits genetic manipulations to be performed. It permits lower cost fermentations to be performed. It permits production of the essential nutrient for subsequent commodity use.

Vitamin prototrophy as a selectable marker

The invention relates to a process for optimizing the production of ethanol by microbial fermentation, particularly microbial fermentation of substrates comprising CO. The process involves monitoring the hydrogen production by the microorganism, determining an optimum hydrogen production and adjusting the substrate supply rate in response to a change in the hydrogen production thereby keeping the hydrogen production within a desired range.

Alcohol production process

The present invention relates to methods for sustaining a microbial culture during periods of limited substrate supply. In accordance with the methods of the invention, a microbial culture comprising carboxydotrophic bacteria can be sustained during periods of limited substrate supply by maintaining the temperature of the microbial culture at a temperature below an optimum operating temperature. Examples of periods of limited substrate supply include when the microbial culture is transported to a remote location or during time when it is stored.

Methods of sustaining culture viability

The invention relates to the production of alcohols by microbial fermentation, particularly to production of alcohols by microbial fermentation of substrates comprising CO. It more particularly relates to processes for the production of alcohols from their corresponding acids in the presence of a substrate comprising CO. In particular embodiments, a fermentation reaction producing acid(s) and optionally alcohol(s) is perturbed such that at least a portion one or more of acid(s) is converted to alcohol.

Microbial alcohol production process

Growing community of inventors

Auckland, New Zealand

Bakir Al-Sinawi