Sanaria Inc.

Protein Potential, LLC

The United States of America, As Represented by the Secretary, Department of Health and Human Services

B Kim Lee Sim

Stephen L Hoffman

Richard E Stafford

Minglin Li

Dennis J Kopecko

Yun Wu

Hong Liang

David L Narum

Steve Fuhrmann

Disclosed is the attenuatedvaccine Ty21a utilized as a vector forand/or enterotoxogenicgenes stably integrated in the Ty21a chromosome. These genes include a heterologousO-antigen biosynthetic gene region that comprises the wzz gene and expressesform 1 O-antigen, as well as a heterologous acid resistance biosynthetic gene system comprising a YbaS gene, which enables increased stability of the Ty21a vector at pH 2.5 relative to Ty21a without the integrated acid resistance biosynthetic gene system.

Live attenuated oral vaccine against shigellosis and typhoid fever

Live attenuated oral vaccine against ETEC and typhoid fever

Disclosed are substantially purifiedsporozoites and preparations ofsporozoites substantially separated from attendant non-sporozoite material, where the preparations ofsporozoites have increasing levels of purity. Vaccines and pharmaceutical compositions comprising purifiedsporozoites are likewise provided. Methods of purifying preparations ofsporozoites are also provided.

Purified plasmodium and vaccine compositions

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Disclosed are substantially purifiedsporozoites and preparations ofsporozoites substantially separated from attendant non-sporozoite material, where the preparations ofsporozoites have increasing levels of purity. Vaccines and pharmaceutical compositions comprising purifiedsporozoites are likewise provided. Methods of purifying preparations of Plasmodium sporozoites are also provided.

Synthetic gene sequences encoding erythrocyte binding protein of a malaria pathogen for the expression of the erythrocyte binding protein. The codon composition of the synthetic gene sequences approximates the mammalian codon composition. The synthetic gene sequences are useful for incorporation into the DNA vaccine vectors, for the incorporation into various expression vectors for production of malaria proteins, or both. The synthetic genes may be modified to avoid post-translational modification of the encoded protein in hosts. Administration of the synthetic gene sequences, or the encoded protein, as an immunization agent is useful for induction of immunity against malaria, treatment of malaria, or both.

Synthetic genes for malarial proteins and methods of use

Growing community of inventors

Gaithersburg, MD, United States of America

B Kim Lee Sim