University of British Columbia

University of Saskatchewan

B Brett Finlay

Markus Stein

Brendan Kenny

Michael Donnenberg

Li-Ching Lai

Andrew A Potter

Robert E W Hancock

Dawn Bowdish

Carrie Melissa Rosenberger

Jon-Paul Steven Powers

Monisha Gough Scott

Rebekah DeVinney

Compositions and methods for stimulating an immune response against a secreted enterohemorragic(EHEC) antigen are disclosed. The compositions comprise EHEC cell culture supernatants.

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The present invention provides a polypeptide, called EspA, which is secreted by pathogenic, such as the enteropathogenic (SPEC) and enterohemorrhagic (EHEC). The invention also provides isolated nucleic acid sequences encoding EspA polypeptide, EspA peptides, a recombinant method for producing recombinant EspA, antibodies which bind to EspA, and a kit for the detection of EspA-producing

A method of identifying a polynucleotide or pattern of polynucleotides regulated by one or more sepsis or inflammatory inducing agents and inhibited by a peptide is described. A method of identifying a pattern of polynucleotide expression for inhibition of an inflammatory or septic response. The method includes contacting cells with LPS, LTA, CpG DNA and/or intact microbe or microbial components in the presence or absence of a cationic peptide; detecting a pattern of polynucleotide expression for the cells in the presence and absence of the peptide, wherein the pattern in the presence of the peptide represents inhibition of an inflammatory or septic response. Also included are compounds and agents identified by the methods of the invention. In another aspect, the invention provides methods and compounds for enhancing innate immunity in a subject.

Effectors of innate immunity determination

Effectors of innate immunity

A polypeptide, called Tir (for translocated intimin receptor, which is secreted by attaching and effacing pathogens, such as the enteropathogenic (EPEC) and enterohemorrhagic (EHEC). These bacterial pathogens inserts their own receptors into mammalian cell surfaces, to which the bacterial pathogen then adheres to trigger additional host signaling events and actin nucleation. Diagnosis of disease caused by pathogeniccan be performed by the use of antibodies which bind to Tir to detect the protein or the use of nucleic acid probes for detection of nucleic acids encoding Tir polypeptide. Isolated nucleic acid sequences encoding Tir polypeptide, Tir peptides, a recombinant method for producing recombinant Tir, antibodies which bind to Tir, and a kit for the detection of Tir-producingare provided. A method of immunizing a host with Tir to induce a protective immune response to Tir or a second polypeptide of interest is also provided. A method for screening for compounds which interfere with the binding of bacterial pathogens to their receptors is further provided.

Host receptor for pathogenic bacteria

The present invention provides a polypeptide, called EspA, which is secreted by pathogenic, such as the enteropathogenic (EPEC) and enterohemorrhagic (EHEC). The invention also provides isolated nucleic acid sequences encoding EspA polypeptide, EspA peptides, a recombinant method for producing recombinant EspA, antibodies which bind to EspA, and a kit for the detection of EspA-producing

The present invention provides the EspA polypeptide, which is secreted by pathogenic such as the enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Diagnosis of disease caused by such pathogenic can be performed by standard techniques, such as those based upon the use of antibodies which bind to EspA to detect the protein, as well as those based on the use of nucleic acid probes for detection of nucleic acids encoding EspA protein. The invention also provides isolated nucleic acid sequences encoding EspA, EspA polypeptide, EspA peptides, a method for producing recombinant EspA, antibodies which bind to EspA, and a kit for the detection of EspA-producing The invention also provides a method of immunizing a host with EspA to induce a protective immune response to EspA.

Growing community of inventors

Richmond, CA, United States of America

B Brett Finlay