Toyota Jidosha Kabushiki Kaisha

Ayumi Koike

Tanetoshi Koyama

Kyozo Ogura

Shusei Obata

Masayoshi Muramatsu

Naoto Shimizu

Yenwin Cho

Tokuzo Nishino

Satoru Nishimura

Shinichi Ohnuma

Takeshi Nakazawa

Choline monooxygenase gene

The present invention provides a polypeptide selected from a polypeptide of subunit (A) or a polypeptide of subunit (B) of a prenyl diphosphate synthetase; a DNA coding for the polypeptide; a recombinant vector comprising the DNA; a transformant transformed with the vector; and a method for preparing an active type prenyl diphosphate synthetase. Also disclosed is a method for preparing an active type enzyme on which a specific property has been conferred, comprising mixing polypeptides of the two subunits of a heterodimeric enzyme, the polypeptides being derived from different organisms and one of the polypeptides having the specific property.

Prenyl diphosphate synthetase genes

Method of making recombinant enzyme

Heptaprenyl diphosphate (HDP)-synthetase derived from Bacillus stearothermophilus which enzymes have the amino acid sequences shown as SEQ ID NOs: 1 to 3; 1 and 2; 2 and 3; or 1 and 3, DNA encoding them, and a method of producing the enzymes. According to the invention it is possible to industrially produce HDP-synthesizing enzyme and HPD.

Heptaprenyl diphosphate-synthetase

DNA encoding heptaprenyl diphosphate synthetase

A mutated farnesyldiphosphate synthase capable of synthesizing geranylgeranyldiphosphate and gene coding for said mutated enzyme, wherein the mutated enzyme is modified from a native farnesyldiphosphate synthase by mutation of a gene coding for a native farnesyldiphosphate synthase.

Mutated farnesyldiphoshate synthase capable of synthesizing

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Toyota, Japan

Ayumi Koike