Fujifilm Corporation

Ayumi Era

Hiroyuki Chiku

Noriyuki Kasagi

Atsuhiko Wada

An object of the present invention is to provide a kit for quantitatively determining a bile acid, in which it is possible to improve measurement accuracy by sufficiently dissociating the bile acid from a polymer component, and to rapidly carry out the quantitative determination of the bile acid with high accuracy under various environments, and a method for quantitatively determining the bile acid. According to the present invention, a kit for quantitatively determining a bile acid in a biological sample, including a compound represented by General Formula (I) defined in the present specification in a dry state; a fluorescent particle that has a first binding substance capable of binding to the bile acid; and a substrate that has a detection region having a second binding substance capable of binding to any one of the bile acid and the first binding substance, is provided.

Kit for quantitatively determining bile acid in biological sample, and method for quantitatively determining bile acid in biological sample

The chromatography method of the present invention includes (a) developing a complex of a test substance and a labeling substance modified with a first binding substance bindable to the test substance, onto an insoluble carrier, (b) causing the complex to be trapped in a reaction site on the insoluble carrier which contains a second binding substance bindable to the test substance or a substance exhibiting binding properties to the first binding substance, (c) washing the insoluble carrier with a washing solution containing at least one of the potassium iodide, urea, and guanidine after the step (b), (d) washing off the washing solution of the step (c) remaining in the insoluble carrier from the insoluble carrier, and (e) amplifying the labeling substance of the complex trapped in the reaction site.

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Kanagawa, Japan

Ayumi Era