Henkel Ag & Company, Kgaa

Ayhan Aydemir

Timothy O'Connell

Brian Laufs

Thomas Weber

Nina Mussmann

Hendrik Hellmuth

Karl-Heinz Maurer

Daniela Herbst

Inken Prueser

Petra Siegert

Ulrich Schwaneberg

Stefan Evers

Marion Merkel

Susanne Tondera

Claudia Lindner

Ronny Martinez

Felix Jakob

Renée Charlott Eichstaedt

Inken Prüser

Timothy O'connell

Renée Charlott EICHSTÄDT

Timothy O'CONNELL

The present disclosure relates to proteases comprising an amino acid sequence which has at least about 70% sequence identity to the amino acid sequence indicated in SEQ ID NO. 1, over the entire length thereof, and in which the amino acids in the positions corresponding to positions 24, 33, 53, 78, 101, 128 and 217 according to SEQ ID NO. 1 are substituted at 24G, 33T, 53G, 78N, 101N, 128A and 217Q, and to the production and use thereof. Said type of proteases have a very good cleaning performance compared to reference proteases.

Protease with improved washing performance

The disclosure relates to proteases, the amino acid sequence of which has been modified in particular with regard to the use thereof in detergents and cleaning agents, all sufficiently similar proteases having a corresponding modification and nucleic acids coding for them. The disclosure further relates to methods and uses of said proteases and to agents containing them, in particular detergents and cleaning agents.

Protease variants having an improved washing performance

The stabilization of a hydrolytic enzyme in a liquid preparation is to be improved through a component that stabilizes the hydrolytic enzyme. This is accomplished by a method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme, comprising the following method steps: a) providing a hydrolytic enzyme (starting enzyme) and a component that stabilizes the hydrolytic enzyme and that comprises a reversible inhibitor of the hydrolytic enzyme; b) changing the amino acid sequence of the hydrolytic enzyme in at least one position, by substitution, deletion or insertion; c) determining the relative activity of the hydrolytic enzyme from step b) and the relative activity of the starting enzyme in a liquid preparation; d) selecting the hydrolytic enzyme that has a reduced relative activity by comparison with the relative activity of the starting enzyme.

Method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme

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Duesseldorf, Germany

Ayhan Aydemir