Hamamatsu-photonics K.k.

Ayano Takeuchi

Masakazu Katsumata

Yuko Kobayashi

Yukiko Sato

Kimiko Kazumura

Hiroshi Satozono

Ryosuke Mochizuki

Lid for a culture vessel

The present invention provides a method for preparing algal cells used for evaluating a toxicity of a chemical substance by delayed luminescence, the method comprising the step of freezing algal cells in a logarithmic growth phase.

Method for preparation of algal cells, and kit for evaluation of toxicity of chemical substance

An object of the present invention is to appropriately and easily evaluate a photosynthetic function of a photosynthetic sample contained in an evaluation sample. An evaluation method for photosynthetic sample according to the present invention is for evaluating a state of a photosynthetic sample with a photosynthetic function based on temporal data of a luminescence amount of delayed luminescence emitted from the photosynthetic sample. First, characteristic values that indicate characteristics are determined for a plurality of time periods in the temporal data. An evaluation value is then computed by weighting the characteristic values. The state of the photosynthetic sample is then evaluated based on the evaluation value.

Evaluation method for evaluating a state of a photosynthesis sample

The present invention provides a method for evaluating the toxicity of a chemical by using an alga, comprising: (a) a thawing step of thawing frozen algal cells by heating, and diluting the obtained suspension of the cells by adding a culture medium thereto; (b) a recovery culture step of culturing the algal cells obtained in the thawing step (a) to allow the algal cells to recover from the effects of freezing and thawing; (c) a confirmation step of collecting a part of the algal cells after the recovery culture step (b), diluting the part of the algal cells by adding a culture medium thereto, and measuring the amount of the luminescence of the delayed luminescence of the algal cells as initial value data; (d) an exposure step of mixing the algal cells after the confirmation step (c) with a solution containing a test substance to prepare an exposure sample, and culturing the exposure sample; (e) a measurement step of measuring the amount of the luminescence of the delayed luminescence of the exposure sample after the exposure step (d) as exposure data; and (f) an evaluation step of calculating an evaluation value based on the initial value data and the exposure data, and evaluating the toxicity of the test substance based on the evaluation value.

Method for evaluating toxicity of chemical using alga

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Hamamatsu, Japan

Ayano Takeuchi