Japan Science and Technology Agency

Centre National De La Recherche Scientifique

Institut Curie

Université Pierre Et Marie Curie

Ayako Yamada

Hirohide Saito

Tan Inoue

Kenichi Yoshikawa

Yusho Kato

Toru Yamanaka

Jean-Louis Viovy

Laurent Malaquin

Stephanie Descroix

Bastien Venzac

Disclosed is a minifluidic device including a matrix, an elongated guiding duct embedded at least in part in the matrix, with at least one port to the outside of the matrix, a movable fiber at least partly contained in the guiding duct, and able to undergo within the guiding duct, and at least along some part of the fiber, at least one action selected among a sliding, or a deformation, or a rotation and at least one of the movable fiber or the guiding duct is elastic or is non linear along at least part of its length, or at least part of the matrix is elastic.

Fluidic devices with at least one actionnable fiber

An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein. The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5' to the ribosome-binding site, and a nucleotide sequence located 5&#x2032; to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site; an mRNA comprising a small RNA-binding site located 3&#x2032; to the start codon, and a nucleotide sequence located 3&#x2032; to the small RNA-binding site, the nucleotide sequence encoding a protein; and a liposome comprising any of these mRNAs encapsulated therein.

Small RNA-dependent translational regulatory system in cell or artificial cell model

Growing community of inventors

Paris, France

Ayako Yamada