Hirosaki University

Ayako Sasaki

Katsuya Yamada

Yuji Otsuka

Tadashi Teshima

Toshihiro Yamamoto

Kouki Ono

Kiyoshi Tone

A cancer cell detection method that makes it possible to perform imaging of cells in a living state and a dual detection method for cancer cells in which the aforementioned method is combined with a pre-existing dyeing method for cytodiagnosis. The method uses living body-derived cells and includes: incubating living cells included in a sample taken from a person together with a fluorescently-labeled L-glucose derivative and detecting the fluorescently-labeled L-glucose derivative that is taken up into the cells; and detecting fluorescence emitted by the L-glucose derivative that is present within the cells while the cells are attached to a thin glass or plastic plate. Also provided is a dual detection method for cancer cells in which the cancer cell detection method that uses living body-derived cells is combined with a dyeing method using cells that are fixed using an alcohol or the like.

Cancer cell detection method using living body-derived cells

Provided is a glucose derivative, which is taken into cells via a membrane sugar transport system and is represented by formula (1). Also provided are an imaging agent and an imaging method for a cell or intracellular molecule using said glucose derivative. Further provided is a method for detecting cancer cells with good accuracy using said glucose derivative and an imaging agent to be used in said method. More specifically provided are D-glucose derivatives and L-glucose derivatives in which glucose is bound to the 7-position of a fluorescent molecular group with a coumarin backbone or a quinolone backbone. Also provided are a cell imaging agent and imaging method using the derivative. A cancer cell imaging agent and imaging method using the L-glucose derivative is also provided. G is a group selected from formulas (G1)-(G4) below.

Glucose derivative, and cell imaging method and imaging agent using said derivative

Provided is a glucose derivative, which is taken into cells via a membrane sugar transport system and is represented by formula (1). Also provided are an imaging agent and an imaging method for a cell or intracellular molecule using said glucose derivative. Further provided are a method for detecting cancer cells with good accuracy using said glucose derivative and an imaging agent to be used in said method. More specifically provided are D-glucose derivatives and L-glucose derivatives in which glucose is bound to the 7-position of a fluorescent molecular group with a coumarin backbone or a quinolone backbone. Also provided are a cell imaging agent and imaging method using the derivative. A cancer cell imaging agent and imaging method using the L-glucose derivative is also provided. G is a group selected from formulas (G1)-(G4) below.

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Hirosaki, Japan

Ayako Sasaki