Nissan Chemical Industries Limited

Nissan Chemical Corporation

Ayako Aihara

Taito Nishino

Hisato Hayashi

Koichiro Saruhashi

Misayo Otani

Takahiro Kishioka

Yoshiomi Hiroi

Takehisa Iwama

Shunsuke Iwamoto

Tatsuro Kanaki

Makiko Umezaki

Keigo Matsumoto

Natsuki Abe

Daisuke Sakuma

Daisuke Hatanaka

Junko Katayama

Hisashi Ishii

Takumi Mikashima

Masahiro Kamaura

Keiichiro Otsuka

The present invention provides a medium composition containing deacylated gellan gum or a salt thereof, and an acidic polysaccharide or a salt thereof capable of maintaining a random coil state in a divalent metal cation medium and cross-linking via a divalent metal ion, and permitting culture of a cell or a tissue in suspension, wherein a concentration of the deacylated gellan gum or a salt thereof in the medium composition is 0.002-0.01 (w/v) %, a concentration of the acidic polysaccharide or a salt thereof is 0.004-0.1 (w/v) %, and a mass ratio of the acidic polysaccharide or a salt thereof to the deacylated gellan gum or a salt thereof is not less than 1. In addition, the present invention provides a method for isolating a cell or tissue from a culture preparation containing the medium composition and cell or tissue, including applying a shear force to the culture preparation.

Culture medium composition for suspension culture allowing easy cell recovery, and cell recovery method

The invention provides a coating agent for a flow passage which comprises a copolymer containing a recurring unit which contains an organic group of the formula (a) wherein Uand Uare defined herein, and a recurring unit which contains an organic group of the formula (b) wherein U, U, U, and Anare defined herein. The invention also provides a flow passage device having the coating agent for a flow passage on at least part of an inner surface of a flow passage, a platelet-producing flow passage device and a method for manufacturing the same.

Coating agent for flow passage

The invention provides a ligand-bonded fiber in which a ligand having affinity for a cell membrane receptor is immobilized on a fiber precursor, and a cell culture substrate capable of repeating ex vivo amplification of a cell expressing a cell membrane receptor by using the ligand-bonded fiber.

Ligand-binding fiber and cell culture substrate using said fiber

The present invention provides a production method of a medium composition, including previously removing divalent metal cations contained in a polymer compound having an anionic functional group, preparing a dry powder of the polymer compound, and mixing the dry powder and the medium, and the like. The present invention also provides a purification method of a polymer compound having an anionic functional group, which includes mixing a suspension of the polymer compound having an anionic functional group in water and a cation exchanger that exchanges a divalent metal cation with a monovalent metal cation to give a polymer compound having an anionic functional group, which shows a reduced amount of contaminating divalent metal cations.

Method for producing culture medium composition

The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues without substantially increasing the viscosity of the solution by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

Culture medium composition

The present invention provides a method of isolating cells or tissues from a culture preparation of the cells or tissues in a medium composition which enables culture of the cells or tissues in suspension, which comprises at least one step selected from the group consisting of the following (A), (B) and (C):

Method for cell recovery

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Shiraoka, Japan

Ayako Aihara